The goal of this project is to enhance genome information using long-read sequencing for inbred mouse strains that serve as disease models, which have been deposited at the NBRP core facility for experimental animal mice (RIKEN BRC) and distributed from there.
The target strains include five inbred disease model strains (
FLS/Shi: nonalcoholic steatohepatitis,
NC/Nga: atopic dermatitis,
STR/OrtCrlj: hereditary osteoarthritis,
Japanese molossinus-derived JF1/Ms: Hirschsprung's disease [Japan Intractable Disease 291]), as well as a healthy control strain (Japanese molossinus-derived MSM/Ms).
These strains have been developed in Japan or are exclusively available to the research community through RIKEN BRC.
This project aims to generate an "accurate genomic sequence" through de novo assembly of long-read sequencing data, independent of reference genomes.
By doing so, it will enable the identification of previously unknown structural variations of 50 bases or more (hereinafter referred to as structural variations).
Additionally, this sequencing initiative will provide the global research community with a high-quality mouse genomic sequence, comparable to and complementary to those produced by ongoing mouse genome projects worldwide.
MoG+ users please acknowledge MoG+ in your publications by mentioning the following information:
MoG+ users please acknowledge MoG+ in your publications by mentioning the following information:
MoG+; https://molossinus.brc.riken.jp/mogplus/
We, Integrated Bioresource Information Division, reserve the right to be the first to publish a genome-wide analysis of the data that we have generated.
BAC clone ordering.