BAC Information

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BAC

Clone NameMSMg01-148K20
Chromosome12 (Build37)
Map Location 25,470,061 - 25,551,598
singlet/doubletdoublet
Overlap geneLOC668719, Mboat2
Upstream geneLOC668471, LOC668703, LOC546534, 9030624G23Rik, EG668710, LOC100042945, EG668711, EG668714, LOC629364, Taf1b, Grhl1, EG629383, Klf11, Cys1, Rrm2
Downstream geneEG629397, C330002I19Rik, Id2, LOC100042973, LOC100042974, LOC668726
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-148K20.T7 (MSMg01no148_k20.b1)MSMg01-148K20.TJ (MSMg01no148_k20.g1)
ACCAG351604AG351605
length720727
length of high quality
(start - end)		249 (71 - 319)650 (78 - 727)
definitionCHROMAT_FILE: MSMg01no148_k20.b1 PHD_FILE: MSMg01no148_k20.b1.phd.1 CHEM: term DYE: big TIME: Tue May 17 19:11:39 2005CHROMAT_FILE: MSMg01no148_k20.g1 PHD_FILE: MSMg01no148_k20.g1.phd.1 CHEM: term DYE: big TIME: Tue May 17 19:11:39 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(25,551,350 - 25,551,598)(25,470,061 - 25,470,710)
sequence
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTCAGGACGCCGCGGAATTCA
CTGAGAGTTGCGAGAGAGGTCAGTCCACAGGAGTAGACTCACAATCCAGC
ACAGAGCACAACAGTACAGTTCTCTCCTGTGCTTCTTGTGGTCATAAAAC
TCTATCCGTGGCTGCCATTCGAAGCTTCAGAATTAGAAACCCAAAAGATA
AGGGAAACAAAAAGGTGCCACTGGCTGGTCAGCATGCAGCAAGTCCCTCC
ATCTGGGGGCAGTAGACACATGAATGCCTCAGCATGGTTGCCCCTGCCTC
GAAAAACTGACTGGCCAGATTTTACGCAGACAACAAAATGGGCCACAACA
ATCGTGCAGTCTTATAGAAGATGGATGAGTATCATACTGGCTGGTTTTGT
GTCAACTTGACACACCCCGCAGTTATCACAGAAAAAGGAGCTTCAGTTGA
GGAAATGCCTCCATGAGATCCAACTGTAAGGCATTTTCTCAATTAGTGAT
CGAGGGGGGAGGGCCCCTTGTGGGTGGGACCATCTCTAGGCTGGTAGTCT
TGGGTTCTATAAGAGAGCAGGCTGAGCAAGCCAGGGGAGGCAAGCCAGTA
AAGAACATCCCTCCATGGCCTCTGCATCAGCTCCTGCTTTCTGACCTGCT
TGAGTTCCAGTCCTGACTTCCTTGGCGATGAACAGCAGCATGGAAGTGTA
AGCTGAATAAACCCTTTCCC

NNNCCGATGCAGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXAGACGTAAATTGTGGCTATGTGT
AAAACAGAGTCCTTGGCCAGCGTCACCCGCTCTCCTCCGTGTTCTCAGGC
TAACTCTGTCAGGAGGACAGTGCTGTCAAACAACTCTCGACCAGATTGTG
GTGGAAGAGCGGGAGACAATGAAGGCTGTGGCTCTTGCTTCTTAGAATGG
AGAAATAGCTCAGTAACATCAAAGGTGATGTCTGTGTATTTATACAAGGG
TTTGTTATTTTATTCTGGAGGTTTTGATTGTTATCTGTGCCATGCCTAAG
CCTTGTATTGAATTCACAAGTGACTTTATACTCAAAAGATGTGGAAAATA
TTTTCAGAGAGCTAAAACTTGCACACGATCATATAACAAGAAATAGCCAA
GATTCCATAAACAGCTCAAGCCCGCATCAGAATGTACACCTGGACCACCA
TCCTTGAGAGCAATGGTTCTCAGCCTTCCTAAGGCTGTGATCCTTTAGTA
CCTCATGCTGTGGTGACCCCCAGCCATAAAGTTGTTTTATTGCTACTTCA
TAACTGTAATTTTGCTACTGTTATGAATCGTAATGTAAATGTTTGATATG
CAGAGTATTTGATATGTGACCCCCAAAAGCGTTTCGACCCACAGGTTGAG
AGCCATTGCTCTGGGGCCCACGGAGGG
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no148_k20.b1 MSMg01no148_k20.b1 CHROMAT_FILE:
MSMg01no148_k20.b1 PHD_FILE: MSMg01no148_k20.b1.phd.1 CHEM: term DYE:
big TIME: Tue May 17 19:11:39 2005
         (249 letters)



>mm_chr12_25551150_25551798
          Length = 649

 Score =  482 bits (249), Expect = e-140
 Identities = 249/249 (100%)
 Strand = Plus / Minus

                                                                       
Query: 1   cagtccacaggagtagactcacaatccagcacagagcacaacagtacagttctctcctgt 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 449 cagtccacaggagtagactcacaatccagcacagagcacaacagtacagttctctcctgt 390

                                                                       
Query: 61  gcttcttgtggtcataaaactctatccgtggctgccattcgaagcttcagaattagaaac 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 389 gcttcttgtggtcataaaactctatccgtggctgccattcgaagcttcagaattagaaac 330

                                                                       
Query: 121 ccaaaagataagggaaacaaaaaggtgccactggctggtcagcatgcagcaagtccctcc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 329 ccaaaagataagggaaacaaaaaggtgccactggctggtcagcatgcagcaagtccctcc 270

                                                                       
Query: 181 atctgggggcagtagacacatgaatgcctcagcatggttgcccctgcctcgaaaaactga 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 269 atctgggggcagtagacacatgaatgcctcagcatggttgcccctgcctcgaaaaactga 210

                    
Query: 241 ctggccaga 249
           |||||||||
Sbjct: 209 ctggccaga 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 13
Number of extensions: 2
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 249
Length of database: 649
Length adjustment: 9
Effective length of query: 240
Effective length of database: 640
Effective search space:   153600
Effective search space used:   153600
X1: 7 (13.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 7 (14.4 bits)
S2: 7 (14.3 bits)

Query= MSMg01no148_k20.g1 MSMg01no148_k20.g1 CHROMAT_FILE:
MSMg01no148_k20.g1 PHD_FILE: MSMg01no148_k20.g1.phd.1 CHEM: term DYE:
big TIME: Tue May 17 19:11:39 2005
         (650 letters)



>mm_chr12_25469861_25470910
          Length = 1050

 Score = 1241 bits (642), Expect = 0.0
 Identities = 648/650 (99%)
 Strand = Plus / Plus

                                                                       
Query: 1   agacgtaaattgtggctatgtgtaaaacagagtccttggccagcgtcacccgctctcctc 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 201 agacgtaaattgtggctatgtgtaaaacagagtccttggccagcgtcacccgctctcctc 260

                                                                       
Query: 61  cgtgttctcaggctaactctgtcaggaggacagtgctgtcaaacaactctcgaccagatt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 261 cgtgttctcaggctaactctgtcaggaggacagtgctgtcaaacaactctcgaccagatt 320

                                                                       
Query: 121 gtggtggaagagcgggagacaatgaaggctgtggctcttgcttcttagaatggagaaata 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 gtggtggaagagcgggagacaatgaaggctgtggctcttgcttcttagaatggagaaata 380

                                                                       
Query: 181 gctcagtaacatcaaaggtgatgtctgtgtatttatacaagggtttgttattttattctg 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 gctcagtaacatcaaaggtgatgtctgtgtatttatacaagggtttgttattttattctg 440

                                                                       
Query: 241 gaggttttgattgttatctgtgccatgcctaagccttgtattgaattcacaagtgacttt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 441 gaggttttgattgttatctgtgccatgcctaagccttgtattgaattcacaagtgacttt 500

                                                                       
Query: 301 atactcaaaagatgtggaaaatattttcagagagctaaaacttgcacacgatcatataac 360
           |||||||||||||||||||||||||||||||||||||||||||| | |||||||||||||
Sbjct: 501 atactcaaaagatgtggaaaatattttcagagagctaaaacttgtatacgatcatataac 560

                                                                       
Query: 361 aagaaatagccaagattccataaacagctcaagcccgcatcagaatgtacacctggacca 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 561 aagaaatagccaagattccataaacagctcaagcccgcatcagaatgtacacctggacca 620

                                                                       
Query: 421 ccatccttgagagcaatggttctcagccttcctaaggctgtgatcctttagtacctcatg 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 621 ccatccttgagagcaatggttctcagccttcctaaggctgtgatcctttagtacctcatg 680

                                                                       
Query: 481 ctgtggtgacccccagccataaagttgttttattgctacttcataactgtaattttgcta 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 681 ctgtggtgacccccagccataaagttgttttattgctacttcataactgtaattttgcta 740

                                                                       
Query: 541 ctgttatgaatcgtaatgtaaatgtttgatatgcagagtatttgatatgtgacccccaaa 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 741 ctgttatgaatcgtaatgtaaatgtttgatatgcagagtatttgatatgtgacccccaaa 800

                                                             
Query: 601 agcgtttcgacccacaggttgagagccattgctctggggcccacggaggg 650
           ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 801 agcgtttcgacccacaggttgagagccattgctctggggcccacggaggg 850


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 31
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 650
Length of database: 1050
Length adjustment: 10
Effective length of query: 640
Effective length of database: 1040
Effective search space:   665600
Effective search space used:   665600
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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