BAC Information

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BAC

Clone NameMSMg01-518K20
Chromosome14 (Build37)
Map Location 29,724,419 - 29,914,926
singlet/doubletdoublet
Overlap geneCacna2d3, Lrtm1
Upstream geneErc2, LOC100040206, Wnt5a, LOC100040218
Downstream genePpia-ps15, EG665431, Selk, Actr8, Il17rb, Chdh, Cacna1d
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-518K20.T7 (MSMg01no518_k20.b1)MSMg01-518K20.TJ (MSMg01no518_k20.g1)
ACCAG590129AG590130
length777778
length of high quality
(start - end)		653 (46 - 698)581 (77 - 657)
definitionCHROMAT_FILE: MSMg01no518_k20.b1 PHD_FILE: MSMg01no518_k20.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:51:09 2005CHROMAT_FILE: MSMg01no518_k20.g1 PHD_FILE: MSMg01no518_k20.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:51:09 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(29,724,419 - 29,725,067)(29,914,346 - 29,914,926)
sequence
CTATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGCGG
AATTCCATGGCACGCGCAAGCATGAAATCGGTGAGGCTCGTGGAGAAGTG
GAGAAGAAACTGCTTTGTTGCAAATAGGGATCTGCCCAATAGCCACAGAT
ACCATCTTAGCTTAGCTAGCAGCTGAGCCCGTGATCCTCTCAGAAGCCTT
GAGTCACTGTGTGCCTCAGTAGATGGAAGACTCCCTGCCATTCAGCACAC
TGAGCTTTAGAATGATCGGTACCGTAGACATGAACTCAAAATCTTCCTGC
TATGTTCCCTACAGAAACTAGGATGGCCACTACTTGTGCTTTCCTACACA
GGGTACTAACTGGTTTCCTTACCTTTATTAAAGCAGGTAAGGGTGTTATA
GTCCCCAGTATTAGCTATCTCCTAATGGAACCTCGGTAGGGTCCTTCACA
TAATACACAACCACCGTCCACAATGACCAGTGCTATAGATGCCAGTGACA
CCTGAGCTCATTCCATCTCATTCTGGTTGTAGGTAGCACAGACCCAATCA
TCTCTCATTTTTTTCTTCAGAGTCTTGGAGAAGGTCTCTGTCTATGATAG
TTGGCACTAAAATACCTGGCATCTCTTTCTCTGAGAGACCAAAGAACAGA
GCTCTCCAGAACCCACTCCACAGAGACGTGGCAGCTATTGCCCCAAGCCC
TCCTCCAGCACCAAGTCAAGCAACTATCTTCCAAAACTGCTGCAGGCAAG
GCTAGACCATGGAGACAGACAACGTTG

CGATTGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXCAGACACCCCAGCAATAATTTCCA
GACACCATGGCCTCTGTTACCATAACGAGCCGTTTTATAATTGGGTTCAC
TTCATTAGCCTGAAGGCTCTCAGCTTGGAAGGGACATGCTTCTCTCCCAG
GGACAGGGGGCTGCACAGCTCCCGGTCTCCAGGGGAGCAGGAGAGCGGGC
TGTGGATCCAGAGCCTGCTTGGCAGATCCTTGTGCTGTGCTGGAAGGTTC
TGTGTCCAATCTGTTCCCCGTTACTCCACGGGACTGAACATGCAAACGGA
CAGGGAGAAGACTGGCTGTGTTTCAAGTGAAATGAGAGTTAGGGGGTTCG
TCAGCATATTGATTGGTGGCCAAAACCAAACATTGTCTATTGTCATTTTA
TGAAGTTCTGATCCTCAGAGAGATCCATCTACCTTCTCTCTTCCTGGAGC
TGTATCGAATGAGCTCCGAGAGTCACCAACCAGCAAGCACAACAGTCTAG
AGCAGTTTCCTCAGAACGGCCTCCTGGGAAGGAGTTTAACCCCATAGCTC
CTGAGTGTCAGGGAAAGTTAGGCACAGGTTTGAGAAAGGCTGGCTGTGTA
TGCTGAAGTAGATTAGCTTCTTGTTGGCTCATTTTTAGTATTTACTTTGG
GTCCATTTTTAAGATCATCTAGATAATGTTTGAATAAAGTGATAAGTGCC
CCAAACATGTGTGGATTTAACTGAGTGG
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no518_k20.b1 MSMg01no518_k20.b1 CHROMAT_FILE:
MSMg01no518_k20.b1 PHD_FILE: MSMg01no518_k20.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:51:09 2005
         (653 letters)



>mm_chr14_29724215_29725267
          Length = 1053

 Score = 1123 bits (581), Expect = 0.0
 Identities = 632/649 (97%)
 Strand = Plus / Plus

                                                                       
Query: 5   gaattccatggcacgcgcaagcatgaaatcggtgaggctcgtggagaagtggagaagaaa 64
           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 205 gaattccatggcacgtgcaagcatgaaatcggtgaggctcgtggagaagtggagaagaaa 264

                                                                       
Query: 65  ctgctttgttgcaaatagggatctgcccaatagccacagataccatcttagcttagctag 124
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 265 ctgctttgttgcaaatagggatctgcccaatagccacagataccatcttagcttagctag 324

                                                                       
Query: 125 cagctgagcccgtgatcctctcagaagccttgagtcactgtgtgcctcagtagatggaag 184
           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 325 cagctgagcccatgatcctctcagaagccttgagtcactgtgtgcctcagtagatggaag 384

                                                                       
Query: 185 actccctgccattcagcacactgagctttagaatgatcggtaccgtagacatgaactcaa 244
           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct: 385 actccctgccattcagcacactgagctttagaatgatcggtaccgtagacatcaactcaa 444

                                                                       
Query: 245 aatcttcctgctatgttccctacagaaactaggatggccactacttgtgctttcctacac 304
           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 445 aatcttcctgctatgttccctacagaaactaggatggccaccacttgtgctttcctacac 504

                                                                       
Query: 305 agggtactaactggtttccttacctttattaaagcaggtaagggtgttatagtccccagt 364
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 505 agggtactaactggtttccttacctttattaaagcaggtaagggtgttatagtccccagt 564

                                                                       
Query: 365 attagctatctcctaatggaacctcggtagggtccttcacataatacacaaccaccgtcc 424
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct: 565 attagctatctcctaatggaacctcggtagggtccttcacataatacacaaccaccatcc 624

                                                                       
Query: 425 acaatgaccagtgctatagatgccagtgacacctgagctcattccatctcattctggttg 484
           |||||||||||||||||||||||||||||  |||||||||||||||||||||||||||||
Sbjct: 625 acaatgaccagtgctatagatgccagtgaggcctgagctcattccatctcattctggttg 684

                                                                       
Query: 485 taggtagcacagacccaatcatctctcatttttttcttcagagtcttggagaaggtctct 544
            | |||||||||||||||||||||||||| ||||||||||||||||  ||||||||||| 
Sbjct: 685 gatgtagcacagacccaatcatctctcatatttttcttcagagtctcagagaaggtctca 744

                                                                       
Query: 545 gtctatgatagttggcactaaaatacctggcatctctttctctgagagaccaaagaacag 604
           |||||||||||||||||||| |||||||||||| ||||||||||||||||||||||||||
Sbjct: 745 gtctatgatagttggcactacaatacctggcatttctttctctgagagaccaaagaacag 804

                                                            
Query: 605 agctctccagaacccactccacagagacgtggcagctattgccccaagc 653
           ||||||||||| ||||||||||||||| |||||||||||||||||||||
Sbjct: 805 agctctccagatcccactccacagagatgtggcagctattgccccaagc 853


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 29
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 653
Length of database: 1053
Length adjustment: 10
Effective length of query: 643
Effective length of database: 1043
Effective search space:   670649
Effective search space used:   670649
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no518_k20.g1 MSMg01no518_k20.g1 CHROMAT_FILE:
MSMg01no518_k20.g1 PHD_FILE: MSMg01no518_k20.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:51:09 2005
         (581 letters)



>mm_chr14_29914146_29915126
          Length = 981

 Score = 1069 bits (553), Expect = 0.0
 Identities = 574/581 (98%)
 Strand = Plus / Minus

                                                                       
Query: 1   cagacaccccagcaataatttccagacaccatggcctctgttaccataacgagccgtttt 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 781 cagacaccccagcaataatttccagacaccatggcctctgttaccataacgagccgtttt 722

                                                                       
Query: 61  ataattgggttcacttcattagcctgaaggctctcagcttggaagggacatgcttctctc 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 721 ataattgggttcacttcattagcctgaaggctctcagcttggaagggacatgcttctctc 662

                                                                       
Query: 121 ccagggacagggggctgcacagctcccggtctccaggggagcaggagagcgggctgtgga 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661 ccagggacagggggctgcacagctcccggtctccaggggagcaggagagcgggctgtgga 602

                                                                       
Query: 181 tccagagcctgcttggcagatccttgtgctgtgctggaaggttctgtgtccaatctgttc 240
           ||||||||||||||||||| |||||||||||||||||||||||||||||| |||||||||
Sbjct: 601 tccagagcctgcttggcagctccttgtgctgtgctggaaggttctgtgtctaatctgttc 542

                                                                       
Query: 241 cccgttactccacgggactgaacatgcaaacggacagggagaagactggctgtgtttcaa 300
           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct: 541 cccgttactccacgggactggacatgcaaacggacagggagaagactggctgtgtttcaa 482

                                                                       
Query: 301 gtgaaatgagagttagggggttcgtcagcatattgattggtggccaaaaccaaacattgt 360
           ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 gtgaaatgagagttaggggtttcgtcagcatattgattggtggccaaaaccaaacattgt 422

                                                                       
Query: 361 ctattgtcattttatgaagttctgatcctcagagagatccatctaccttctctcttcctg 420
           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct: 421 ctattgtcattttatgaagttctgattctcagagagatccatctaccttctctcttcctg 362

                                                                       
Query: 421 gagctgtatcgaatgagctccgagagtcaccaaccagcaagcacaacagtctagagcagt 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 gagctgtatcgaatgagctccgagagtcaccaaccagcaagcacaacagtctagagcagt 302

                                                                       
Query: 481 ttcctcagaacggcctcctgggaaggagtttaaccccatagctcctgagtgtcagggaaa 540
           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 ttcctcagaacgacctcctgggaaggagtttaaccccatagctcctgagtgtcagggaaa 242

                                                    
Query: 541 gttaggcacaggtttgagaaaggctggctgtgtatgctgaa 581
           |||||||||||||||||||| ||||||||||||||||||||
Sbjct: 241 gttaggcacaggtttgagaagggctggctgtgtatgctgaa 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 27
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 581
Length of database: 981
Length adjustment: 10
Effective length of query: 571
Effective length of database: 971
Effective search space:   554441
Effective search space used:   554441
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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