BAC Information

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BAC

Clone NameMSMg01-521K20
Chromosome14 (Build37)
Map Location 49,259,566 - 49,433,019
singlet/doubletdoublet
Overlap geneOtx2, LOC100041864
Upstream geneKtn1, 4930447J18Rik, Map1lc3-ps2, Peli2, EG624367, LOC100041831, 6720456H20Rik, 4930572G02Rik
Downstream geneLOC666972, Exoc5, 4932432K03Rik, LOC218997, Nat12, 1700011H14Rik, Slc35f4, 3632451O06Rik, LOC667006
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-521K20.T7 (MSMg01no521_k20.b1)MSMg01-521K20.TJ (MSMg01no521_k20.g1)
ACCAG592417AG592418
length760748
length of high quality
(start - end)		552 (52 - 603)579 (78 - 656)
definitionCHROMAT_FILE: MSMg01no521_k20.b1 PHD_FILE: MSMg01no521_k20.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:55:05 2005CHROMAT_FILE: MSMg01no521_k20.g1 PHD_FILE: MSMg01no521_k20.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:55:05 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(49,259,566 - 49,260,113)(49,432,441 - 49,433,019)
sequence
NNNTTCCTATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XCGCGGAATTCAAGGCTTATCTAGTTGTTTTTGGCTCCAAATAAAATACA
ACAGTAGGACTGGGTTACAGATCAGTAGCAGGACATTTGACTAACGTGCA
CAAAGCCCTGGGTTCAATCCCAGATACTGTGAGGGTTGGGCGGAGGGGTT
CAGAGGCAGTTTCTTCTCTATTAATATTAGAGTCGTCTATTAGAGGGCTG
GAGAGATGGCTCAATGGTTAAGAGCACTTGCTGCTCTGGCTCAAGACCTG
GGCTCAGTTCTCAGCATTCGCATAGTCACTTGTAACTCCAGTTTCTGGGG
ATTGCACTCTTCTCCGACCTTCTCAGGTACCAGCAAGTACAGGATACACA
TGCACACATGCAGACAAACACCCATACTCATACAACAGAAATATAAAAAA
GAATACTTAGTAGATGTTGGGTGTAAATTTTAATGGCTCAGCGGAATGCT
AGTATTTAATATTCTAAATGCTTTCCTTCTTGGCCGAATGTTTTTCTGAA
GTGTGCTTACTATCTCTGAGAAAGTCCTTCGTGGTCACTTTTACTGCCTG
ATGTATTCTGTTGGCTCTCTCTCTTTACTGCTGCCCTAAGTGGCAAGCAG
CAGCCTGGTAGCTTGGAGGTATCCACAGCTGTGCATCTTGTGTATTGGAC
AAACACCCCACAGCANGCTATGGAGACAGCTGTCCGTGCTTCTTCGGGAA
CCAAGAACAN

NCCACGATGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXAGGAGGAAAGAAATGACACGATG
CGGAGCTGGCAAAGGGCCGCCTCTAGTGGGAACAGATGCCCAACAAATTT
GGCGAGTAGTTTTGTTCTCTTCTATCTGCTGCGGTTCACAATCCTAATGA
ATGTTATGTTTAAACAACATGAATAAAGATAGAACGAGAATGAATTCAAC
TCCGGTTTGAGCCGCAGAATAAAAATGAGCAAACTGTATTATCTGCTGCC
ACCTAATCTGGGTTGAATTTCCTCTGTATCTTTTTTTGTTTGTTGAAGAA
TCCTTTTCTTGAGTCTCCTTGGCCATTGACAAGGTCTTTTGAGATGAATA
TGAAAAGATAGATTTAAAGGTTAAGGCCAGGGTCAGATGGTTGCAGATGT
TTTGTGAGAGATTGTTCGGTTCAGTTTGGGGTACTCCTCCTGACTAAGGT
ATTTCAAACTGCCAGACTCATGGCCAGCCACCACTGAAGACTGTTGTTTG
TCTCACGATTGATCCAACACAAACGAGGAATAGGAGTGTTTGTTTTGTTT
CTTTCTTTCTTCAGGGGATAATTCCCTGCGCATGCCCCCTGGGGGCTGGG
GGGAAGGCAAAGACTGTCTAAAGCCAGAGAAGGTGTTGTTTTTATTCTTA
GAATGTAGACTATTTTAGGTTTTGTTCTTCAAATGATCATGGGAGACC
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no521_k20.b1 MSMg01no521_k20.b1 CHROMAT_FILE:
MSMg01no521_k20.b1 PHD_FILE: MSMg01no521_k20.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:55:05 2005
         (552 letters)



>mm_chr14_49259362_49260313
          Length = 952

 Score = 1052 bits (544), Expect = 0.0
 Identities = 547/548 (99%)
 Strand = Plus / Plus

                                                                       
Query: 5   gaattcaaggcttatctagttgtttttggctccaaataaaatacaacagtaggactgggt 64
           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 205 gaattcaaggcttagctagttgtttttggctccaaataaaatacaacagtaggactgggt 264

                                                                       
Query: 65  tacagatcagtagcaggacatttgactaacgtgcacaaagccctgggttcaatcccagat 124
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 265 tacagatcagtagcaggacatttgactaacgtgcacaaagccctgggttcaatcccagat 324

                                                                       
Query: 125 actgtgagggttgggcggaggggttcagaggcagtttcttctctattaatattagagtcg 184
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 325 actgtgagggttgggcggaggggttcagaggcagtttcttctctattaatattagagtcg 384

                                                                       
Query: 185 tctattagagggctggagagatggctcaatggttaagagcacttgctgctctggctcaag 244
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 385 tctattagagggctggagagatggctcaatggttaagagcacttgctgctctggctcaag 444

                                                                       
Query: 245 acctgggctcagttctcagcattcgcatagtcacttgtaactccagtttctggggattgc 304
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 445 acctgggctcagttctcagcattcgcatagtcacttgtaactccagtttctggggattgc 504

                                                                       
Query: 305 actcttctccgaccttctcaggtaccagcaagtacaggatacacatgcacacatgcagac 364
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 505 actcttctccgaccttctcaggtaccagcaagtacaggatacacatgcacacatgcagac 564

                                                                       
Query: 365 aaacacccatactcatacaacagaaatataaaaaagaatacttagtagatgttgggtgta 424
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 565 aaacacccatactcatacaacagaaatataaaaaagaatacttagtagatgttgggtgta 624

                                                                       
Query: 425 aattttaatggctcagcggaatgctagtatttaatattctaaatgctttccttcttggcc 484
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 625 aattttaatggctcagcggaatgctagtatttaatattctaaatgctttccttcttggcc 684

                                                                       
Query: 485 gaatgtttttctgaagtgtgcttactatctctgagaaagtccttcgtggtcacttttact 544
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 685 gaatgtttttctgaagtgtgcttactatctctgagaaagtccttcgtggtcacttttact 744

                   
Query: 545 gcctgatg 552
           ||||||||
Sbjct: 745 gcctgatg 752


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 26
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 552
Length of database: 952
Length adjustment: 10
Effective length of query: 542
Effective length of database: 942
Effective search space:   510564
Effective search space used:   510564
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no521_k20.g1 MSMg01no521_k20.g1 CHROMAT_FILE:
MSMg01no521_k20.g1 PHD_FILE: MSMg01no521_k20.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:55:05 2005
         (579 letters)



>mm_chr14_49432241_49433219
          Length = 979

 Score = 1104 bits (571), Expect = 0.0
 Identities = 577/579 (99%)
 Strand = Plus / Minus

                                                                       
Query: 1   aggaggaaagaaatgacacgatgcggagctggcaaagggccgcctctagtgggaacagat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 779 aggaggaaagaaatgacacgatgcggagctggcaaagggccgcctctagtgggaacagat 720

                                                                       
Query: 61  gcccaacaaatttggcgagtagttttgttctcttctatctgctgcggttcacaatcctaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 719 gcccaacaaatttggcgagtagttttgttctcttctatctgctgcggttcacaatcctaa 660

                                                                       
Query: 121 tgaatgttatgtttaaacaacatgaataaagatagaacgagaatgaattcaactccggtt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 659 tgaatgttatgtttaaacaacatgaataaagatagaacgagaatgaattcaactccggtt 600

                                                                       
Query: 181 tgagccgcagaataaaaatgagcaaactgtattatctgctgccacctaatctgggttgaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 599 tgagccgcagaataaaaatgagcaaactgtattatctgctgccacctaatctgggttgaa 540

                                                                       
Query: 241 tttcctctgtatctttttttgtttgttgaagaatccttttcttgagtctccttggccatt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 539 tttcctctgtatctttttttgtttgttgaagaatccttttcttgagtctccttggccatt 480

                                                                       
Query: 301 gacaaggtcttttgagatgaatatgaaaagatagatttaaaggttaaggccagggtcaga 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 479 gacaaggtcttttgagatgaatatgaaaagatagatttaaaggttaaggccagggtcaga 420

                                                                       
Query: 361 tggttgcagatgttttgtgagagattgttcggttcagtttggggtactcctcctgactaa 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 419 tggttgcagatgttttgtgagagattgttcggttcagtttggggtactcctcctgactaa 360

                                                                       
Query: 421 ggtatttcaaactgccagactcatggccagccaccactgaagactgttgtttgtctcacg 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 359 ggtatttcaaactgccagactcatggccagccaccactgaagactgttgtttgtctcacg 300

                                                                       
Query: 481 attgatccaacacaaacgaggaataggagtgtttgttttgtttctttctttcttcagggg 540
           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||| ||
Sbjct: 299 attgatccaacacaaatgaggaataggagtgtttgttttgtttctttctttcttcagtgg 240

                                                  
Query: 541 ataattccctgcgcatgccccctgggggctggggggaag 579
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 239 ataattccctgcgcatgccccctgggggctggggggaag 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 28
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 979
Length adjustment: 10
Effective length of query: 569
Effective length of database: 969
Effective search space:   551361
Effective search space used:   551361
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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