BAC Information

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BAC

Clone NameMSMg01-401E04
Chromosome16 (Build37)
Map Location 16,808,024 - 16,990,848
singlet/doubletdoublet
Overlap geneSpag6, EG665562, EG436405, Vpreb1, Top3b, Ppm1f, LOC100042783, EG623519, Mapk1
Upstream genePrkdc, 2410018G20Rik, Cebpd, 2310008H04Rik, LOC100043266, LOC224010, D16Ertd727e, Pkp2, EG433003, Yars2, Dnm1l, LOC665743, Fgd4, Olfr19, 4933404G15Rik
Downstream geneYpel1, Ppil2, 2610318N02Rik, Sdf2l1, LOC623568, Ccdc116, 1810015A11Rik, Ube2l3, Gm603, Hic2, D16Bwg1494e, Pik4ca, Serpind1, Snap29, Crkl, Aifm3, Lztr1, Thap7, 4930451C15Rik, P2rxl1, Slc7a4, Smpd4, Pcqap, EG433050, Map1lc3-ps8, EG665892, LOC100042830, Klhl22, Scarf2, Car15, Dgcr2, Tssk1, Tssk2, Es2el, Gscl, Slc25a1, Vpreb2
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-401E04.T7 (MSMg01no401_e04.b1)MSMg01-401E04.TJ (MSMg01no401_e04.g1)
ACCAG500688AG500689
length751756
length of high quality
(start - end)
548 (74 - 621)466 (81 - 546)
definitionCHROMAT_FILE: MSMg01no401_e04.b1 PHD_FILE: MSMg01no401_e04.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 18:14:50 2005CHROMAT_FILE: MSMg01no401_e04.g1 PHD_FILE: MSMg01no401_e04.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 18:14:50 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(16,990,285 - 16,990,848)(16,808,024 - 16,808,480)
sequence
NNTATCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCG
CGGAATTCAGGTCCTCACAACACATAAAAAGTCAGGTGTAACTGTGAATA
CCTGTAACCCTAGCACTTAGAATGTGGACACCAACTGACAACCAAGTATT
CAAATACAAGAGTCTACAGAGGACATTTTCATTCAAACCACCACAGGGAC
CCTGGAGAGAATAATCTAGTCTGTGGACTTTTTAATGCATTTAAAGCAAC
AGAACCCTTGCCTTATGTTGCTGGTCCTAATAATCATGTGTGAAGGTTAA
TAGATGACTGAAAGGAAAACTTTTAGGTGGGAGAGAAGGAAAGACCAAAG
TGCTGATGCTTCAAAAAGGAGGACTGTCATCCGTAAGGTTTTGTTCGTAA
GGTTTCCCGGGCGTGTAGGAAAGGTGACAGTTTGAAAACCCCAGACAGAA
CACAATCCTTTCAATTTTCTTTGTCAACAAGGAACCTTTCACTTTGTTGT
TTGTAACTTTCTCTAGTGGTTCTGCTCACATTCCATAAAGCCTCACGTCA
GAACCCTCCTTGCATCTTTCTCCTCACACAGAGTGGGCCATTTCAAAGGT
CAGGCTGTCAGTCTTTACTAGTGGAACATTCCAAAGCGAAAAGGAATAAA
AGCAAAGGCACACAGATCATTCTAAGGTTGCACAGCAAGTATTATTCTGT
GAAAGAAATGTTTCCAATACTTATTCCTGTATCCGTGTGAGGACCTAACT
G
CTTTCGATGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCTACACTGATTAACAAAGG
TGGCTGCAACAGTGTGCAATCCCAAAACCCGTGCGGGTTTTTGTTTATCT
TCGCCATTTTGTCAGGGATAAAATGGTTTTTTATTGTTTGCATTTCCCTA
GTTGCTAGGATTGATGAACACCTTCTTCTTCTTCTTCTTCTTCTTCTTCT
TCTTCTTCTTCTTCTTCTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC
CTCCTCCTCCTCCTCCTCCTCCTTCTTTTTTTCTTCCTTCTTCTTCTTCT
TTCTTCTTTCTTCTTCCTTCTTTTCTTTCTTCTCTTTTCTTTTCTTCTTT
CTTCTTCTTCTTTCTTCTTTTCTTCTTCTTTTCTTCTTCTTCTTCTTTCT
TCCTTCTTCTTTCCTTCTTCTTCTTCTTTCTTCCTTCTTCTTCTTCTTCT
TTCTTCTTCCTTCTTTTCTTTCTTCTTCTTCTCTTTTCTTTTCTTCTTCT
TTCTTCTTCTTTCTTTTCTTCTTCCTCTTTTCTTCCTCTTTCTTCCTTCT
TTCCCTCTTCATCTACCCTTCTTCTTCTTCCCCTTCTTCCTTCAATTCCT
CTTCTTTTGCTGCTGCTGCGGCCGCTGCTGCTGCCTGCTGTTGCTGCTGC
TTCCTTTGCTTCCCCCTGTTACCTCTGATTTTGGCCCCCCTGAAAAAATT
CCTGCN
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no401_e04.b1 MSMg01no401_e04.b1 CHROMAT_FILE:
MSMg01no401_e04.b1 PHD_FILE: MSMg01no401_e04.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 18:14:50 2005
         (548 letters)



>mm_chr16_16990191_16991138
          Length = 948

 Score =  936 bits (484), Expect = 0.0
 Identities = 538/568 (94%), Gaps = 24/568 (4%)
 Strand = Plus / Minus

                                                                       
Query: 1   ataaaaagtcaggtgtaactgtgaatacctgtaaccctagcacttagaatgtggacacca 60
           ||||||||||||||||||| ||||||||||||||||||||| ||||||| ||||||||||
Sbjct: 658 ataaaaagtcaggtgtaaccgtgaatacctgtaaccctagcgcttagaaggtggacacca 599

                                                                       
Query: 61  actgacaaccaagtattcaaatacaagagtctacagaggacattttcattcaaaccacca 120
           ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct: 598 actgacaaccaagtattcagatacaagagtctacagaggacattttcattcaaaccacca 539

                                                                       
Query: 121 cagggaccctggagagaataatctagtctgtggactttttaatgcatttaaagcaacaga 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 538 cagggaccctggagagaataatctagtctgtggactttttaatgcatttaaagcaacaga 479

                                                                       
Query: 181 acccttgccttatgttgctggtcctaataatcatgtgtgaaggttaatagatgactgaaa 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 478 acccttgccttatgttgctggtcctaataatcatgtgtgaaggttaatagatgactgaaa 419

                                                                       
Query: 241 ggaaaac----ttttaggtgggagagaaggaaagaccaaagtgctgatgcttcaaaaagg 296
           |||||||        |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 418 ggaaaacaaaa----aggtgggagagaaggaaagaccaaagtgctgatgcttcaaaaagg 363

                                                                       
Query: 297 aggactgtcatccgtaaggttttgttcgtaaggtttcccg--------ggc---gt--g- 342
           ||||||||||||||||||||||||||| ||||||||||||        |||   ||  | 
Sbjct: 362 aggactgtcatccgtaaggttttgttcataaggtttcccgtacgtgaaggctctgtcggc 303

                                                                       
Query: 343 --taggaaaggtgacagtttgaaaaccccagacagaacacaatcctttcaattttctttg 400
             | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 302 ccttggaaaggtgacagtttgaaaaccccagacagaacacaatcctttcaattttctttg 243

                                                                       
Query: 401 tcaacaaggaacctttcactttgttgtttgtaactttctctagtggttctgctcacattc 460
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 242 tcaacaaggaacctttcactttgttgtttgtaactttctctagtggttctgctcacattc 183

                                                                       
Query: 461 cataaagcctcacgtcagaaccctccttgcatctttctcctcacacagagtgggccattt 520
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 182 cataaagcctcacgtcagaaccctccttgcatctttctcctcacacagagtgggccattt 123

                                       
Query: 521 caaaggtcaggctgtcagtctttactag 548
           ||||||||||||||||||||||||||||
Sbjct: 122 caaaggtcaggctgtcagtctttactag 95


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 24
Number of extensions: 3
Number of successful extensions: 3
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 948
Length adjustment: 10
Effective length of query: 538
Effective length of database: 938
Effective search space:   504644
Effective search space used:   504644
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)
Query= MSMg01no401_e04.g1 MSMg01no401_e04.g1 CHROMAT_FILE:
MSMg01no401_e04.g1 PHD_FILE: MSMg01no401_e04.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 18:14:50 2005
         (466 letters)



>mm_chr16_16807824_16808689
          Length = 866

 Score =  685 bits (354), Expect = 0.0
 Identities = 436/478 (91%), Gaps = 33/478 (6%)
 Strand = Plus / Plus

                                                                       
Query: 1   tctacactgattaacaaaggtggctgcaacagtgtgcaatcccaaaacccgtgcgggttt 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 201 tctacactgattaacaaaggtggctgcaacagtgtgcaatcccaaaacccgtgcgggttt 260

                                                                       
Query: 61  ttgtttatcttcgccattttgtcagggataaaatggttttttattgtttgcatttcccta 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 261 ttgtttatcttcgccattttgtcagggataaaatggttttttattgtttgcatttcccta 320

                                                                       
Query: 121 gttgctaggattgatgaacaccttcttcttcttcttcttcttcttcttcttcttcttctt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 gttgctaggattgatgaacaccttcttcttcttcttcttcttcttcttcttcttcttctt 380

                                                                       
Query: 181 cttcttctccttcttcttcttcttcttcttcttcttcttcctcctc--ct-------cct 231
           |||||||| ||||||||||||||||||||||||||||||| || ||  ||       |||
Sbjct: 381 cttcttcttcttcttcttcttcttcttcttcttcttcttcttcttcttcttcttcttcct 440

                                                                       
Query: 232 cctcctcctccttctttttttcttccttcttcttcttctttcttctttcttcttccttct 291
           |||||||||||| ||      | |||| || || || ||  ||||   || |||||||||
Sbjct: 441 cctcctcctcctcct------cctcctcctcctcctcct--cttc---ct-cttccttct 488

                                                                       
Query: 292 tttctttcttctcttttcttttcttctt---tcttcttcttctttcttcttttcttcttc 348
           |  ||| |||||||||||||||||||||   |||||||||||||||||||||||||||||
Sbjct: 489 t--ctt-cttctcttttcttttcttcttctttcttcttcttctttcttcttttcttcttc 545

                                                                       
Query: 349 ttttcttcttcttcttctttcttccttcttctttccttcttcttcttctttcttccttct 408
           ||||||||||||||||   |||||||||||||||||||||||||||||||||||||||||
Sbjct: 546 ttttcttcttcttctt---tcttccttcttctttccttcttcttcttctttcttccttct 602

                                                                     
Query: 409 tcttcttcttctttcttcttccttcttttctttcttcttcttctcttttcttttcttc 466
           ||||||||||   |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 603 tcttcttctt---tcttcttccttcttttctttcttcttcttctcttttcttttcttc 657



 Score =  132 bits (68), Expect = 6e-35
 Identities = 129/152 (84%), Gaps = 11/152 (7%)
 Strand = Plus / Plus

                                                                       
Query: 143 ttcttcttcttcttcttcttc-ttcttcttcttcttcttcttcttctccttcttcttctt 201
           ||||||||||| ||||||||| ||||||||||||||||||||||||| ||||||||||||
Sbjct: 677 ttcttcttctt-ttcttcttctttcttcttcttcttcttcttcttcttcttcttcttctt 735

                                                                       
Query: 202 cttcttcttcttcttcttc--ctcctcctcctcctcctcctccttctttttttcttcctt 259
           |||||||||||||||||||  || || || || || || || |||||| |   |||| ||
Sbjct: 736 cttcttcttcttcttcttctgctgctgctgctgctgctgctgcttcttctg--cttc-tt 792

                                           
Query: 260 cttcttcttct--ttcttct--ttcttcttcc 287
           || ||||||||  |||||||  |||| |||||
Sbjct: 793 ctgcttcttctgcttcttctgcttctgcttcc 824



 Score =  128 bits (66), Expect = 9e-34
 Identities = 75/78 (96%), Gaps = 2/78 (2%)
 Strand = Plus / Plus

                                                                       
Query: 142 cttcttcttcttcttcttc-ttcttcttcttcttcttcttcttcttctccttcttcttct 200
           ||||||||| ||||||||| |||||||||||||||||||||||||||| |||||||||||
Sbjct: 679 cttcttctt-ttcttcttctttcttcttcttcttcttcttcttcttcttcttcttcttct 737

                             
Query: 201 tcttcttcttcttcttct 218
           ||||||||||||||||||
Sbjct: 738 tcttcttcttcttcttct 755



 Score =  122 bits (63), Expect = 5e-32
 Identities = 75/79 (94%), Gaps = 3/79 (3%)
 Strand = Plus / Plus

                                                                       
Query: 143 ttcttcttcttcttcttcttcttcttc-ttcttcttcttcttcttctccttcttcttctt 201
           ||||| ||||||||||| ||||||||| ||||||||||||||||||| ||||||||||||
Sbjct: 672 ttctt-ttcttcttctt-ttcttcttctttcttcttcttcttcttcttcttcttcttctt 729

                              
Query: 202 cttcttcttcttcttcttc 220
           |||||||||||||||||||
Sbjct: 730 cttcttcttcttcttcttc 748



 Score =  118 bits (61), Expect = 7e-31
 Identities = 76/81 (93%), Gaps = 4/81 (4%)
 Strand = Plus / Plus

                                                                       
Query: 142 cttcttc-ttcttcttcttcttcttcttcttcttc-ttcttcttcttctccttcttcttc 199
           ||||||| ||||| ||||||||||| ||||||||| ||||||||||||| ||||||||||
Sbjct: 664 cttcttctttctt-ttcttcttctt-ttcttcttctttcttcttcttcttcttcttcttc 721

                                
Query: 200 ttcttcttcttcttcttcttc 220
           |||||||||||||||||||||
Sbjct: 722 ttcttcttcttcttcttcttc 742



 Score =  116 bits (60), Expect = 3e-30
 Identities = 75/80 (93%), Gaps = 4/80 (5%)
 Strand = Plus / Plus

                                                                       
Query: 143 ttcttcttcttc-ttcttcttcttcttcttcttcttcttc-ttcttctccttcttcttct 200
           |||||||||||| ||||| ||||||||||| ||||||||| ||||||| |||||||||||
Sbjct: 659 ttcttcttcttctttctt-ttcttcttctt-ttcttcttctttcttcttcttcttcttct 716

                               
Query: 201 tcttcttcttcttcttcttc 220
           ||||||||||||||||||||
Sbjct: 717 tcttcttcttcttcttcttc 736



 Score =  112 bits (58), Expect = 4e-29
 Identities = 76/82 (92%), Gaps = 5/82 (6%)
 Strand = Plus / Plus

                                                                       
Query: 142 cttc-ttcttcttcttc-ttcttcttcttcttcttcttcttcttctt-ctccttcttctt 198
           |||| |||||||||||| ||||| ||||||||||| ||||||||||| || |||||||||
Sbjct: 654 cttctttcttcttcttctttctt-ttcttcttctt-ttcttcttctttcttcttcttctt 711

                                 
Query: 199 cttcttcttcttcttcttcttc 220
           ||||||||||||||||||||||
Sbjct: 712 cttcttcttcttcttcttcttc 733



 Score = 83.9 bits (43), Expect = 2e-20
 Identities = 70/79 (88%)
 Strand = Plus / Plus

                                                                       
Query: 142 cttcttcttcttcttcttcttcttcttcttcttcttcttcttcttctccttcttcttctt 201
           ||||||||||||||||||||||||||||||||||||||||| || || || || || || 
Sbjct: 715 cttcttcttcttcttcttcttcttcttcttcttcttcttctgctgctgctgctgctgctg 774

                              
Query: 202 cttcttcttcttcttcttc 220
           || |||||||| |||||||
Sbjct: 775 ctgcttcttctgcttcttc 793



 Score = 78.1 bits (40), Expect = 1e-18
 Identities = 71/82 (86%), Gaps = 3/82 (3%)
 Strand = Plus / Plus

                                                                       
Query: 142 cttcttcttcttcttcttcttcttcttcttcttcttcttcttcttctccttct--t-ctt 198
           ||||||||||||||||||||||||||||||||||| || || || || || ||  | |||
Sbjct: 721 cttcttcttcttcttcttcttcttcttcttcttctgctgctgctgctgctgctgctgctt 780

                                 
Query: 199 cttcttcttcttcttcttcttc 220
           ||||| |||||||| |||||||
Sbjct: 781 cttctgcttcttctgcttcttc 802


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 583
Number of extensions: 76
Number of successful extensions: 76
Number of sequences better than 10.0: 1
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 18
Length of query: 466
Length of database: 866
Length adjustment: 10
Effective length of query: 456
Effective length of database: 856
Effective search space:   390336
Effective search space used:   390336
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)