BAC Information

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BAC

Clone NameMSMg01-457B01
Chromosome16 (Build37)
Map Location 73,607,994 - 73,786,168
singlet/doubletdoublet
Overlap genenone
Upstream geneRobo1, LOC100039310, LOC100039318, LOC100039326
Downstream geneRobo2
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-457B01.T7 (MSMg01no457_b01.b1)MSMg01-457B01.TJ (MSMg01no457_b01.g1)
ACCAG543220AG543221
length728749
length of high quality
(start - end)		617 (51 - 667)434 (83 - 516)
definitionCHROMAT_FILE: MSMg01no457_b01.b1 PHD_FILE: MSMg01no457_b01.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 19:29:07 2005CHROMAT_FILE: MSMg01no457_b01.g1 PHD_FILE: MSMg01no457_b01.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 19:29:07 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(73,607,994 - 73,608,606)(73,785,735 - 73,786,168)
sequence
NGGGATCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
CGCGGAATTCATACCAAATGTGCTGCTATCAGATATCATGCCCCCATGTT
TCATTCAATTCAAAAGACATTGATGGTGAGAATTTGGCAATATTTAAGAT
TAGAACACTTGTAACTTTTCTTTACTACTAGACAAAGGTAACACTTGAGG
ATATAACTTTATACTCCCAAATAATTACTTGAGCTTCCTCATTTTTGCTG
AGAATCTAAGGGAGTCTGTGTAGGAATGCACAGTAACGTTTTGGGATGGT
GGGAAAAAATAAAAAATGTTCAGTTACACTAGGCTCTGTAAGTAGGGAAT
CTGCATTTTATATGGTAGCTCAGGGGGTTGGAAGCGTTAGGTTCATTGCC
TGAAAGAGATGAAAATGTAGACAGTATTGAGAAAGTTGGGAGTACAAGCT
AAAGTGATCACAATAAAGGTTGGATTCTTTCGGGTTTATCACCACATTGC
CTAGTATGATGGGCTTTCTGAAAATTAAAATAGGAAGTTGACATTCACAT
TAGGTGCTATTTACATGAACTTGACATAAACTAGAGACACTTGGGAAGAG
AGAGAGCCACAACTGAGAACATGCCTCCATCGGAATGAGCTACATGAAGA
TCTACAGGTCATTTTCTGGGTTACTAGCAGATGGTATAGGTCTCTGCCTA
CGGTGAGTCACACCCCCTCTGGGTAGGN

NNNCCCCGATGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCTTTCACATCCTATTTG
AGATTTGAAGCAAATGATTCTGGCATTTGCTAGCTCTAGAATACTGTTTA
GTAGCTAACTTACCTTAATAAAACTTCTTTTTGATACAGGATATTTTACA
ATATTAATGCCATCTGAAAATTTTAACTTGATACGCAAGAGAGATTTGAG
ACAAGGAAAAAATGGGGCATTCTAGAAAGTTAACCAAGGCTGAATTTATT
TTTTACGAAATGTCTGGTATATATATGCCAGCATATAAATTTAAAGATAT
TTGTCATTCTCCAAATTAGATAGATGATTTATATATAGATAACATTACAA
TAGATAATATGTTATAGTCTGTATATACTTCGTGTGTGTTTAAAGACATT
GTTCATTGTTCCCATTGTGTGTGTGTGCATGTGCACATTTAAAATTATCT
TCGAGCAATTGTCCCAATAGTTAATATGTTTGGATCTAGGAAAGGATACC
TACATTGCTATAGAACATATGTTATCTATTCTTTGCTTTCTCCTGTCCTG
GGCTGTTTTTTAGAACCACGTTATTGTTGTCCTCTTTTTAAAAGTTTGTC
TACTTGGTACCTCTCCAATTTTTGTGGGTGTTTTGTTCCCTCTTTCTTTT
GTGCTCTCCTTTCTCTGTTTTCTTCTTTCTCCCTTTTTTGTTATTCCTC
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no457_b01.b1 MSMg01no457_b01.b1 CHROMAT_FILE:
MSMg01no457_b01.b1 PHD_FILE: MSMg01no457_b01.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 19:29:07 2005
         (617 letters)



>mm_chr16_73607790_73608806
          Length = 1017

 Score = 1170 bits (605), Expect = 0.0
 Identities = 611/613 (99%)
 Strand = Plus / Plus

                                                                       
Query: 5   gaattcataccaaatgtgctgctatcagatatcatgcccccatgtttcattcaattcaaa 64
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 205 gaattcataccaaatgtgctgctatcagatatcatgcccccatgtttcattcaattcaaa 264

                                                                       
Query: 65  agacattgatggtgagaatttggcaatatttaagattagaacacttgtaacttttcttta 124
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 265 agacattgatggtgagaatttggcaatatttaagattagaacacttgtaacttttcttta 324

                                                                       
Query: 125 ctactagacaaaggtaacacttgaggatataactttatactcccaaataattacttgagc 184
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 325 ctactagacaaaggtaacacttgaggatataactttatactcccaaataattacttgagc 384

                                                                       
Query: 185 ttcctcatttttgctgagaatctaagggagtctgtgtaggaatgcacagtaacgttttgg 244
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 385 ttcctcatttttgctgagaatctaagggagtctgtgtaggaatgcacagtaacgttttgg 444

                                                                       
Query: 245 gatggtgggaaaaaataaaaaatgttcagttacactaggctctgtaagtagggaatctgc 304
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 445 gatggtgggaaaaaataaaaaatgttcagttacactaggctctgtaagtagggaatctgc 504

                                                                       
Query: 305 attttatatggtagctcagggggttggaagcgttaggttcattgcctgaaagagatgaaa 364
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 505 attttatatggtagctcagggggttggaagcgttaggttcattgcctgaaagagatgaaa 564

                                                                       
Query: 365 atgtagacagtattgagaaagttgggagtacaagctaaagtgatcacaataaaggttgga 424
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 565 atgtagacagtattgagaaagttgggagtacaagctaaagtgatcacaataaaggttgga 624

                                                                       
Query: 425 ttctttcgggtttatcaccacattgcctagtatgatgggctttctgaaaattaaaatagg 484
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 625 ttctttcgggtttatcaccacattgcctagtatgatgggctttctgaaaattaaaatagg 684

                                                                       
Query: 485 aagttgacattcacattaggtgctatttacatgaacttgacataaactagagacacttgg 544
           ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 685 aaggtgacattcacattaggtgctatttacatgaacttgacataaactagagacacttgg 744

                                                                       
Query: 545 gaagagagagagccacaactgagaacatgcctccatcggaatgagctacatgaagatcta 604
           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct: 745 gaagagagagagccacaactgagaacatgcctccatcggaatgagctacatgcagatcta 804

                        
Query: 605 caggtcattttct 617
           |||||||||||||
Sbjct: 805 caggtcattttct 817


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 29
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 617
Length of database: 1017
Length adjustment: 10
Effective length of query: 607
Effective length of database: 1007
Effective search space:   611249
Effective search space used:   611249
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no457_b01.g1 MSMg01no457_b01.g1 CHROMAT_FILE:
MSMg01no457_b01.g1 PHD_FILE: MSMg01no457_b01.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 19:29:07 2005
         (434 letters)



>mm_chr16_73785535_73786368
          Length = 834

 Score =  824 bits (426), Expect = 0.0
 Identities = 432/434 (99%)
 Strand = Plus / Minus

                                                                       
Query: 1   tctttcacatcctatttgagatttgaagcaaatgattctggcatttgctagctctagaat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 634 tctttcacatcctatttgagatttgaagcaaatgattctggcatttgctagctctagaat 575

                                                                       
Query: 61  actgtttagtagctaacttaccttaataaaacttctttttgatacaggatattttacaat 120
           |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct: 574 actgtttagtagctaacttaccttaataaaacttatttttgatacaggatattttacaat 515

                                                                       
Query: 121 attaatgccatctgaaaattttaacttgatacgcaagagagatttgagacaaggaaaaaa 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 514 attaatgccatctgaaaattttaacttgatacgcaagagagatttgagacaaggaaaaaa 455

                                                                       
Query: 181 tggggcattctagaaagttaaccaaggctgaatttattttttacgaaatgtctggtatat 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 454 tggggcattctagaaagttaaccaaggctgaatttattttttacgaaatgtctggtatat 395

                                                                       
Query: 241 atatgccagcatataaatttaaagatatttgtcattctccaaattagatagatgatttat 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 394 atatgccagcatataaatttaaagatatttgtcattctccaaattagatagatgatttat 335

                                                                       
Query: 301 atatagataacattacaatagataatatgttatagtctgtatatacttcgtgtgtgttta 360
           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 334 atatagataacattacaatagataatatgttatagtctgtatatacttcgtgtgcgttta 275

                                                                       
Query: 361 aagacattgttcattgttcccattgtgtgtgtgtgcatgtgcacatttaaaattatcttc 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 274 aagacattgttcattgttcccattgtgtgtgtgtgcatgtgcacatttaaaattatcttc 215

                         
Query: 421 gagcaattgtccca 434
           ||||||||||||||
Sbjct: 214 gagcaattgtccca 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 23
Number of extensions: 2
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 434
Length of database: 834
Length adjustment: 9
Effective length of query: 425
Effective length of database: 825
Effective search space:   350625
Effective search space used:   350625
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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