BAC Information

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BAC

Clone NameMSMg01-424F18
Chromosome2 (Build37)
Map Location 20,634,139 - 20,823,339
singlet/doubletdoublet
Overlap geneEtl4, Arhgap21
Upstream geneLOC100041302, LOC433406, LOC654360, LOC667609
Downstream geneLOC433408, LOC675912, C130046B21Rik, 4933434I06Rik, Thnsl1, Gpr158
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-424F18.T7 (MSMg01no424_f18.b1)MSMg01-424F18.TJ (MSMg01no424_f18.g1)
ACCAG518297AG518298
length761744
length of high quality
(start - end)		575 (44 - 618)245 (80 - 324)
definitionCHROMAT_FILE: MSMg01no424_f18.b1 PHD_FILE: MSMg01no424_f18.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 18:45:18 2005CHROMAT_FILE: MSMg01no424_f18.g1 PHD_FILE: MSMg01no424_f18.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 18:45:18 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(20,822,844 - 20,823,339)(20,634,139 - 20,634,380)
sequence
ATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGCGGAA
TTCCTGCCTCTTCATTAACCTGTTCCGAGAAACTGAGGCACTAACACAAG
TTTGATACTAGGAAATAAGGAAACAAGAAGCAAAAATTGGTTATTAGATT
ATGCAAAGTAAAATACCTAGTTGTTGAGTATGGACTTTCCCTCGTAGCTC
CCTCTGTGCTTGCCCCTTCCGCATCCTCCTCCTCTCTTTTTGTAGAATCA
GGATTTCTTTATGTTGACTTGGCTGCTTTTGGACTCCTGGGCTCAAGTGA
CTCCTGTCTCAGCCTCCCAAGTCAAGTGCTAACTACAAATCCACCCCATT
TACCTATCATTATGTTTTTGAAATGATTTCTATGAATTCTTCTCAGAGAA
AAGATGATGAAGGACAGAAAATGCGTTCTGTGACTGAGTTTTTCATTAAG
TAGGTACCGGGTACATAGTAGGAGCTTGTATGCACAGTGCCTGTTGGGTA
AAGGGAGGATGCTGACACCTTTTGTCATGGCCACCATGGTCTGTTCCTAA
GTGACTGCTCTGTGTCTTCCTTCTCCCTCCCCATAGCCCGGGTAACTCAG
GGTTAGTGGTACCTAGTGATTTAGAAACCATATTTCAAATAACTAAGAAA
GTCTTTAAGAAAATAACAAAAATAGGGCAATCTTGGACTTACATAAAAAT
GTATACATATTCTATAAAATAAAACAGTANGTGAAAAGTTTAAAAGAGAA
ACTTGATGCTN

NCCCATGAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAATGGTTGTACTCAGCCCAC
TCAGTACAAGAACACCTACAAGAAAGAAACCTTCTGCTTACCCACAACCC
CTCAGCTCCAGCCACCATCGGATAGATAGGCTAGAGGGGTTACAGGTGAC
CCTTGCAGAAGAGCCTAAGGAGAGAGAAGGGCGTCGTCATACATGGGCTG
CTCTTGTGTGATTTGAGCAGGCTTTCTTCTGCAATCTGATTTCTTGCCTC
TAAGAACTGGGATCAAAAGCATATATGATTCAGTGTGTTATATTAACTTT
GTGTTTCAATGTGTATGGAGGCAAGCTTTTTGTTTTTTGTTTTTTTAAAA
TGCACTGTGGGAGGAAAAGAATGCATTGTTAAACATACTGGGCTTGGTCT
TTTTGATATTCTTTCCTATATTTTGTATACATTTTGTTTCCCTTGCTGAG
CATTTGATATCAAAGAAAATAATTTTTAAGTGTACATTCATTGGATAAAG
AGGAATGTTTAAACAAAGTAAACAATTAAATATTTAAATATTAACCAAGT
CTCTTGAAGGGATTGTAAATACTGAAGTTTTGATGCTTTTTAGAGTGCTC
TCTTTCTGTACTCTGAAGCTTGGAAAGAAGTTCTTGTTACCTGATATCCC
TGGCTGGACTTTAACGCTGCCAGAATAACATGTTTCCTAGGTAA
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no424_f18.b1 MSMg01no424_f18.b1 CHROMAT_FILE:
MSMg01no424_f18.b1 PHD_FILE: MSMg01no424_f18.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 18:45:18 2005
         (575 letters)



>mm_chr2_20822365_20823339
          Length = 975

 Score =  897 bits (464), Expect = 0.0
 Identities = 488/496 (98%)
 Strand = Plus / Minus

                                                                       
Query: 80  acaagaagcaaaaattggttattagattatgcaaagtaaaatacctagttgttgagtatg 139
           ||||||||||||||||||||||||||||||||||||||||||| | ||||||||||||||
Sbjct: 975 acaagaagcaaaaattggttattagattatgcaaagtaaaatatcgagttgttgagtatg 916

                                                                       
Query: 140 gactttccctcgtagctccctctgtgcttgccccttccgcatcctcctcctctctttttg 199
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 915 gactttccctcgtagctccctctgtgcttgccccttccgcatcctcctcctctctttttg 856

                                                                       
Query: 200 tagaatcaggatttctttatgttgacttggctgcttttggactcctgggctcaagtgact 259
           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 855 tagaatcaggatttccttatgttgacttggctgcttttggactcctgggctcaagtgact 796

                                                                       
Query: 260 cctgtctcagcctcccaagtcaagtgctaactacaaatccaccccatttacctatcatta 319
           ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 795 cctgtctcagcctcccaagtcaagtgctaactacaaatccaccccatttacttatcatta 736

                                                                       
Query: 320 tgtttttgaaatgatttctatgaattcttctcagagaaaagatgatgaaggacagaaaat 379
           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 735 tgtttttgaaacgatttctatgaattcttctcagagaaaagatgatgaaggacagaaaat 676

                                                                       
Query: 380 gcgttctgtgactgagtttttcattaagtaggtaccgggtacatagtaggagcttgtatg 439
           |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 675 gcgtcctgtgactgagtttttcattaagtaggtaccgggtacatagtaggagcttgtatg 616

                                                                       
Query: 440 cacagtgcctgttgggtaaagggaggatgctgacaccttttgtcatggccaccatggtct 499
           ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||| ||
Sbjct: 615 cacagtgcctgttgggtaaggggaggatgctgacaccttttgtcatggccaccatggcct 556

                                                                       
Query: 500 gttcctaagtgactgctctgtgtcttccttctccctccccatagcccgggtaactcaggg 559
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 555 gttcctaagtgactgctctgtgtcttccttctccctccccatagcccgggtaactcaggg 496

                           
Query: 560 ttagtggtacctagtg 575
           ||||||||||||||||
Sbjct: 495 ttagtggtacctagtg 480


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 24
Number of extensions: 2
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 575
Length of database: 975
Length adjustment: 10
Effective length of query: 565
Effective length of database: 965
Effective search space:   545225
Effective search space used:   545225
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no424_f18.g1 MSMg01no424_f18.g1 CHROMAT_FILE:
MSMg01no424_f18.g1 PHD_FILE: MSMg01no424_f18.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 18:45:18 2005
         (245 letters)



>mm_chr2_20633939_20634583
          Length = 645

 Score =  460 bits (238), Expect = e-134
 Identities = 241/242 (99%)
 Strand = Plus / Plus

                                                                       
Query: 1   caatggttgtactcagcccactcagtacaagaacacctacaagaaagaaaccttctgctt 60
           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct: 201 caatggttgtactcagcccactcagtacaagaacacctacaagaaagaaaccgtctgctt 260

                                                                       
Query: 61  acccacaacccctcagctccagccaccatcggatagataggctagaggggttacaggtga 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 261 acccacaacccctcagctccagccaccatcggatagataggctagaggggttacaggtga 320

                                                                       
Query: 121 cccttgcagaagagcctaaggagagagaagggcgtcgtcatacatgggctgctcttgtgt 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 cccttgcagaagagcctaaggagagagaagggcgtcgtcatacatgggctgctcttgtgt 380

                                                                       
Query: 181 gatttgagcaggctttcttctgcaatctgatttcttgcctctaagaactgggatcaaaag 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 gatttgagcaggctttcttctgcaatctgatttcttgcctctaagaactgggatcaaaag 440

             
Query: 241 ca 242
           ||
Sbjct: 441 ca 442


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 10
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 245
Length of database: 645
Length adjustment: 9
Effective length of query: 236
Effective length of database: 636
Effective search space:   150096
Effective search space used:   150096
X1: 7 (13.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 7 (14.4 bits)
S2: 7 (14.3 bits)


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