BAC Information

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BAC

Clone NameMSMg01-435L20
Chromosome2 (Build37)
Map Location 20,665,231 - 20,849,862
singlet/doubletdoublet
Overlap geneEtl4, Arhgap21
Upstream geneLOC100041302, LOC433406, LOC654360, LOC667609
Downstream geneLOC433408, LOC675912, C130046B21Rik, 4933434I06Rik, Thnsl1, Gpr158
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-435L20.T7 (MSMg01no435_l20.b1)MSMg01-435L20.TJ (MSMg01no435_l20.g1)
ACCAG526974AG526975
length811802
length of high quality
(start - end)		422 (42 - 463)430 (70 - 499)
definitionCHROMAT_FILE: MSMg01no435_l20.b1 PHD_FILE: MSMg01no435_l20.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 19:00:35 2005CHROMAT_FILE: MSMg01no435_l20.g1 PHD_FILE: MSMg01no435_l20.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 19:00:36 2005
singlet/doubletdoubletdoublet
BLAST hitpluralunique
Map location.(20,849,448 - 20,849,862)(20,665,231 - 20,665,660)
sequence
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGCGGAATT
CAGTTACTAATGCCTGTCTGTATACCCATGGACTGAGAACCAAAATTTCT
AGAAAAATTTGTTTTTTACATTTAGTTTGGAATTTTTGTTTCAAAGTTGT
GTAATCCTTCTTTTTTAAAAATAAAATACCCGGAGTATACATCTCAATGA
GAATAATTTTTCTTTTCAAGTGTGATACATGTCTTGCAATGTTCTAGTAA
TATAGCAACTGTTCATTGAACTTCTGGCCTCCCATGTACTGGTGTTTAAT
TATGATTAATGTTTAAATATTTATTGCAGTAACATATCTTCAAGAAGAAC
ACTTTGCCCTTTATAAGTAGAATAAGGTCATCAGTTCTTTAGGAGACAGT
CCCTTAGTGTGCTCACCTTATTCAAAATACCTTATAAAATTTTCTTTTGT
TAAAAGCTTAAGCTATAAACCATTATAACAAGAGCGAACAATTTCAGCTT
CCCCTGATGTTTTACTCATGGGTGTTTGCCCGGGCTTTCTTTTTATAAAT
TCTGAATTCATTTCTTAACTTGTTTACAAACTTTGCCCTTTTTTTCCCCT
CCCCCCTTGTTTTCTCTTATTCTCTTCCCCCTTCCCCACTCCTTTTTTCC
TTTTTTTTTTTTCCTTTCTTCTTATCCCTTTTATTTTTCTTCCCCCTTCT
TCTATTTCCCCCATTTTTTTTACTTCTGTTTTCTCCCTCTTTCACCCCCC
ATTTATTTTTTTTTATTCCCCCCGCTATATTTTTCTACCTTATGACCCAC
CCTATCTCCCN

AATGTTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXCTTACGGGGGCAGCCGTCCAATGGCTATTCC
TGGCAATGCCACCATCCCCAGGGACAGACTCTCGAGCCTGCCAGTATCCA
GATCCATCTCCCCAAGCCCCAGTGCCATTTTGGAAAGAAGAGATGTCAAA
CCGGATGAAGACATGAGCAGCAAAAACCTGGTTATGTTCAGAAATGAGGG
TTTCTATGCTGACCCTTACCTCTATCATGAAGGACGAATGAGCATAGCCT
CATCCCATGGCAGCCACCCTTTGGATGTCCCAGATCATGTCATTGCGTAT
CACCGCACAGCGATCCGGTCAGCGAGTGCTTACTGTAGCCCTTCCTTGCA
AGCAGAAATGCATATGGAGCAGTCGTTATACAGACAAAAGTCAAGGAAAT
ACCCTGATAGCCATTTGCCTACCTTGGGCTCCAAAACGCCCCCAGCCTCT
CCACACCGTGTGGGTGACTTAAGAATGATAGACCTGCACCCGCATCTTAA
CACACATGGGCCCCCTCCCACTCTTGGAGCAGACCGGGTCTTCCCCCAGT
GCCCGTTTCTTTATAAAAAAACCCGTCACCCCGGGGGAAATCCTTAAAAC
TTCGTAACCCCTTCCTCTATCATTCCTCCCCTGTACCACTATGTGCTCCT
CTCCCCTTTGTTCAAATAAAGCGTTTCTTTACACCATATTTGTTTTTATT
CCCTACTAATGACAAATCTTCCGCCTTATTTAATATATCCTCTATCTCCC
AG
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no435_l20.b1 MSMg01no435_l20.b1 CHROMAT_FILE:
MSMg01no435_l20.b1 PHD_FILE: MSMg01no435_l20.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 19:00:35 2005
         (422 letters)



>mm_chr2_20849241_20850062
          Length = 822

 Score =  764 bits (395), Expect = 0.0
 Identities = 410/415 (98%)
 Strand = Plus / Minus

                                                                       
Query: 8   ttcagttactaatgcctgtctgtatacccatggactgagaaccaaaatttctagaaaaat 67
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 622 ttcagttactaatgcctgtctgtatacccatggactgagaaccaaaatttctagaaaaat 563

                                                                       
Query: 68  ttgttttttacatttagtttggaatttttgtttcaaagttgtgtaatccttcttttttaa 127
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 562 ttgttttttacatttagtttggaatttttgtttcaaagttgtgtaatccttcttttttaa 503

                                                                       
Query: 128 aaataaaatacccggagtatacatctcaatgagaataatttttcttttcaagtgtgatac 187
           ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 502 aaataaaatacccggagtatacatctcaatgagaataatttttcttttcaaatgtgatac 443

                                                                       
Query: 188 atgtcttgcaatgttctagtaatatagcaactgttcattgaacttctggcctcccatgta 247
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 442 atgtcttgcaatgttctagtaatatagcaactgttcattgaacttctggcctcccatgta 383

                                                                       
Query: 248 ctggtgtttaattatgattaatgtttaaatatttattgcagtaacatatcttcaagaaga 307
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 382 ctggtgtttaattatgattaatgtttaaatatttattgcagtaacatatcttcaagaaga 323

                                                                       
Query: 308 acactttgccctttataagtagaataaggtcatcagttctttaggagacagtcccttagt 367
           ||||||||||||||||||||||||||||| ||||||||||||||||||||| ||||||||
Sbjct: 322 acactttgccctttataagtagaataaggccatcagttctttaggagacagccccttagt 263

                                                                  
Query: 368 gtgctcaccttattcaaaataccttataaaattttcttttgttaaaagcttaagc 422
           ||||| |||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct: 262 gtgcttaccttattcaaaataccttatacaattttcttttgttaaaagcttaagc 208


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 22
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 422
Length of database: 822
Length adjustment: 9
Effective length of query: 413
Effective length of database: 813
Effective search space:   335769
Effective search space used:   335769
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no435_l20.g1 MSMg01no435_l20.g1 CHROMAT_FILE:
MSMg01no435_l20.g1 PHD_FILE: MSMg01no435_l20.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 19:00:36 2005
         (430 letters)



>mm_chr2_20665031_20665860
          Length = 830

 Score =  808 bits (418), Expect = 0.0
 Identities = 427/430 (99%)
 Strand = Plus / Plus

                                                                       
Query: 1   cttacgggggcagccgtccaatggctattcctggcaatgccaccatccccagggacagac 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 201 cttacgggggcagccgtccaatggctattcctggcaatgccaccatccccagggacagac 260

                                                                       
Query: 61  tctcgagcctgccagtatccagatccatctccccaagccccagtgccattttggaaagaa 120
           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct: 261 tctcgagcctgccagtatccagatccatctccccaagccccagcgccattttggaaagaa 320

                                                                       
Query: 121 gagatgtcaaaccggatgaagacatgagcagcaaaaacctggttatgttcagaaatgagg 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 gagatgtcaaaccggatgaagacatgagcagcaaaaacctggttatgttcagaaatgagg 380

                                                                       
Query: 181 gtttctatgctgacccttacctctatcatgaaggacgaatgagcatagcctcatcccatg 240
           |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 gtttctatgccgacccttacctctatcatgaaggacgaatgagcatagcctcatcccatg 440

                                                                       
Query: 241 gcagccaccctttggatgtcccagatcatgtcattgcgtatcaccgcacagcgatccggt 300
           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 441 gcggccaccctttggatgtcccagatcatgtcattgcgtatcaccgcacagcgatccggt 500

                                                                       
Query: 301 cagcgagtgcttactgtagcccttccttgcaagcagaaatgcatatggagcagtcgttat 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 501 cagcgagtgcttactgtagcccttccttgcaagcagaaatgcatatggagcagtcgttat 560

                                                                       
Query: 361 acagacaaaagtcaaggaaataccctgatagccatttgcctaccttgggctccaaaacgc 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 561 acagacaaaagtcaaggaaataccctgatagccatttgcctaccttgggctccaaaacgc 620

                     
Query: 421 ccccagcctc 430
           ||||||||||
Sbjct: 621 ccccagcctc 630


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 21
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 830
Length adjustment: 9
Effective length of query: 421
Effective length of database: 821
Effective search space:   345641
Effective search space used:   345641
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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