BAC Information

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BAC

Clone NameMSMg01-278E21
Chromosome4 (Build37)
Map Location 66,511,567 - 66,618,192
singlet/doubletdoublet
Overlap genenone
Upstream geneAstn2, LOC667499, Tlr4
Downstream geneLOC100039379, Hmgb1-rs18, LOC433715
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-278E21.T7 (MSMg01no278_e21.b1)MSMg01-278E21.TJ (MSMg01no278_e21.g1)
ACCAG415963AG415964
length698705
length of high quality
(start - end)
306 (379 - 684)324 (382 - 705)
definitionCHROMAT_FILE: MSMg01no278_e21.b1 PHD_FILE: MSMg01no278_e21.b1.phd.1 CHEM: term DYE: big TIME: Tue May 17 21:18:36 2005CHROMAT_FILE: MSMg01no278_e21.g1 PHD_FILE: MSMg01no278_e21.g1.phd.1 CHEM: term DYE: big TIME: Tue May 17 21:18:36 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(66,511,567 - 66,511,872)(66,617,869 - 66,618,192)
sequence
TCATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCGCGG
AATTCTTGTCTGCCCAGAGTAAACTCTGCATAAATTTCACCATTGAAGAT
TAGTGATAGAAGAGAACTCCATTCGCTCTTTCTTCTGGCTTTATTTTTTA
TTTTTAATGCTGTCTGATTGCCCAAGGTATGTATGGAGGGTGTACACAGA
CGGTACACAGACCTAAGTCAGGTGTCTAAGCATCCCAGGAACTTCCCTTC
CAATATTCTTTTCTGAGCATATGCCCTCAGTTAGTTTTCCTCTTCATATG
ATCTGTGCTCCTGTTTATACCAAACTCTCGGCTCTGGCAGCATCCTCGTC
CAAAATTTTAAGTTCAGCCCCCCCCACTGGTCACATACCACCACCATTTT
CTACTCTTTATACTTTCTTTCCCTGATTACATTCCAATAGTGTGTAGGCA
TGAACACATGTGCACACATACACACATGTGCAGATTATAGTCCACTTGTA
GCAATAAGAGGATTCTCAGTACAATTCGTGGGAGTTGGATTTCTCCTGCC
ACCACATAGGTACAATTAATCCCAGTACTCGGGAGGCAGAGGCAGGCAGA
TTCCTGAGTTCAAGGCCAGCCTGGTCTAAAAAGTGAGTTCCAGGACAGCC
AGAGCTACACAGAAAAACCCCGTCTCAAAAAACCAAATAAAAAAAAAA
CGATGACXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXCATCAACTGTGGACCATGTGTTCA
AATATATGTTTAAAGGATCCACTCTCATTCAAACCACATCTATATAGGAA
TATATCACATTAAATACCTTCTAAGGATAATTAAGATTAAAATGTTAGAA
GCATATTTGAATTTTTATAATACTTCTTAGCATTTTTAAATTGCATATGT
ATTTAAACTAAAAATAAAATACAACTTTACACTAGCCTGCTCAAACTTAA
AAATTAAGCATATTCATAATATATGGCCAAGGCAGCTATTATTTTCTTTA
AATTTACTTATTTGTGAAACCCGGCTNACCCACCTAGCATCGTTTCCCTG
AGGTCATGCTTCTTGTCGCAACAGAATAGAATTCAAGTGCGTGTGTGTTG
GCTCCAAGGCTTTACAAGAGTAAGTCCTTGATTTCTTTGGCCCACAGCAA
TATCAAATTCTGTGTATGAGAAAAGGCTTTCAATATAAACTGTTATGATG
CATACTAACACAAAAATTTCTGAAGATATGAGAATTCTAACCCGGTGATG
GAGGTCAGTGAGTAGGTGGGGAAAATGAAAGAAGTGCGAGAGGACAGAAG
CCATCAGTGAGGTGAGAAGAGAGCTTCCCCCAAGGAGAGAGGGAAGGAAG
GTTAA
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no278_e21.b1 MSMg01no278_e21.b1 CHROMAT_FILE:
MSMg01no278_e21.b1 PHD_FILE: MSMg01no278_e21.b1.phd.1 CHEM: term DYE:
big TIME: Tue May 17 21:18:36 2005
         (306 letters)



>mm_chr4_66511367_66512072
          Length = 706

 Score =  592 bits (306), Expect = e-173
 Identities = 306/306 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   ggtcacataccaccaccattttctactctttatactttctttccctgattacattccaat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 201 ggtcacataccaccaccattttctactctttatactttctttccctgattacattccaat 260

                                                                       
Query: 61  agtgtgtaggcatgaacacatgtgcacacatacacacatgtgcagattatagtccacttg 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 261 agtgtgtaggcatgaacacatgtgcacacatacacacatgtgcagattatagtccacttg 320

                                                                       
Query: 121 tagcaataagaggattctcagtacaattcgtgggagttggatttctcctgccaccacata 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 tagcaataagaggattctcagtacaattcgtgggagttggatttctcctgccaccacata 380

                                                                       
Query: 181 ggtacaattaatcccagtactcgggaggcagaggcaggcagattcctgagttcaaggcca 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 ggtacaattaatcccagtactcgggaggcagaggcaggcagattcctgagttcaaggcca 440

                                                                       
Query: 241 gcctggtctaaaaagtgagttccaggacagccagagctacacagaaaaaccccgtctcaa 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 441 gcctggtctaaaaagtgagttccaggacagccagagctacacagaaaaaccccgtctcaa 500

                 
Query: 301 aaaacc 306
           ||||||
Sbjct: 501 aaaacc 506


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 17
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 306
Length of database: 706
Length adjustment: 9
Effective length of query: 297
Effective length of database: 697
Effective search space:   207009
Effective search space used:   207009
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)
Query= MSMg01no278_e21.g1 MSMg01no278_e21.g1 CHROMAT_FILE:
MSMg01no278_e21.g1 PHD_FILE: MSMg01no278_e21.g1.phd.1 CHEM: term DYE:
big TIME: Tue May 17 21:18:36 2005
         (324 letters)



>mm_chr4_66617669_66618392
          Length = 724

 Score =  596 bits (308), Expect = e-175
 Identities = 320/324 (98%)
 Strand = Plus / Minus

                                                                       
Query: 1   acctagcatcgtttccctgaggtcatgcttcttgtcgcaacagaatagaattcaagtgcg 60
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct: 524 acctagcatcgtttccctgaggtcatgcttcttgtcgcaacagaatagaattcaagtgtg 465

                                                                       
Query: 61  tgtgtgttggctccaaggctttacaagagtaagtccttgatttctttggcccacagcaat 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 464 tgtgtgttggctccaaggctttacaagagtaagtccttgatttctttggcccacagcaat 405

                                                                       
Query: 121 atcaaattctgtgtatgagaaaaggctttcaatataaactgttatgatgcatactaacac 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 404 atcaaattctgtgtatgagaaaaggctttcaatataaactgttatgatgcatactaacac 345

                                                                       
Query: 181 aaaaatttctgaagatatgagaattctaacccggtgatggaggtcagtgagtaggtgggg 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
Sbjct: 344 aaaaatttctgaagatatgagaattctaacccggtgatggaggtcagtgagtaggtggga 285

                                                                       
Query: 241 aaaatgaaagaagtgcgagaggacagaagccatcagtgaggtgagaagagagcttccccc 300
           ||||||||||||||| |||||||||||||||| |||||||||||||||||||||||||||
Sbjct: 284 aaaatgaaagaagtgagagaggacagaagccaccagtgaggtgagaagagagcttccccc 225

                                   
Query: 301 aaggagagagggaaggaaggttaa 324
           ||||||||||||||||||||||||
Sbjct: 224 aaggagagagggaaggaaggttaa 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 14
Number of extensions: 2
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 324
Length of database: 724
Length adjustment: 9
Effective length of query: 315
Effective length of database: 715
Effective search space:   225225
Effective search space used:   225225
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)