BAC Information

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BAC

Clone NameMSMg01-484L20
Chromosome5 (Build37)
Map Location 18,840,227 - 19,022,265
singlet/doubletdoublet
Overlap geneMagi2
Upstream geneGnai1, LOC100041818, LOC668167, 4921504A21Rik
Downstream genenone
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-484L20.T7 (MSMg01no484_l20.b1)MSMg01-484L20.TJ (MSMg01no484_l20.g1)
ACCAG564354AG564355
length731764
length of high quality
(start - end)		584 (76 - 659)691 (74 - 764)
definitionCHROMAT_FILE: MSMg01no484_l20.b1 PHD_FILE: MSMg01no484_l20.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:06:25 2005CHROMAT_FILE: MSMg01no484_l20.g1 PHD_FILE: MSMg01no484_l20.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:06:25 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(18,840,227 - 18,840,810)(19,021,575 - 19,022,265)
sequence
NCAACCTATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXC
GCGGAATTCTTTGGCTTCACAAAATTTTACAGATACATTTGTTCAATATG
CCTAGTGTTTATTTTCTCCTCAAGGGTAGATATAACATAAAAAGCTCTGA
GAGTTGAGGAGTTCGTAGATCAATCCTCCTATTTGAAAATTAATCATCAC
TCTGAATAGGAGACCTGTTGGATATTGTGTATACATTTAGTTTCAAAATG
ATTTACTGTTTCTCTTAATGTTACCTTGTACTGATTGAAACTGCAGAACT
AATGGATTACTGGAGAGTGAAGCTTCCCCTCTTATCTCAATCTTGGCTGC
TGATGTAGATTTTTGTGTAGCCTGTGAAGTTTGCAAAACAGCAGGCTAGT
CATTAGAAATTGCAGAGTACAGATCAAATTCAACAGTCAATCACTCTACT
ATAACAATTTTATTAAGCTCTAACTGAATCTATAATAAATATACTATTGT
AAGTCTCCAGTATGTTACCTTGTGAATTAAAACCATGCTGAACAGATCCA
GTTTTCATACTCTGATTGTACAAATCCTTTGAACATTAACATCCAAATAG
GGGAATGCCAGGGCCAGGAAGCAGGAGTGGGTGGGTTGGGGAACAGGGGA
GGGAGGAGGGGATAGAGGATTTTCTCGCTCCCCTTTTTTCTCCTCTCCTT
TTTTTTCTCTTTCGCGCCTTTTTCTCCACCT

ATGATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXAAGTTTGGGCCCAGCTTTGCTGATGAG
TAATTAGAAAAAGTCTTACTGTCTCTCTGCTTTTCTGAGTAGAGAATCTT
AGAAATTAATTCTGTGGAGGAATGATGAAATCAAAAGATTCTAACATTTC
CAGAAGGAGACGGCTTTTTGGATTCCAACAAAGTCCACTGGCTTCCATTC
AGATGAATGCAATTTCATATTCCCATTTGAGAATAACTATGAGACAAGAT
TAGTTTTAAAAGTGTTTATTTTAAAAAATTTCCAACATATTGAAGAGAGT
GATTGAAACATGTAGCTGTGCTTTCAACAGGAGATTATGATGGTAGCAAG
GGATGCCTGGACCATGGCAGGCCACTGCTGCCAGCACCACCTTCCTTTAC
ATTAGAGTTGGGTACCAGATCAGAGATATTTTTAGCTAACAACAGACTAT
TATATAGCTAGTACCCAATGATGTTTTCTAAAGGAATCAAATAATTGATA
AATGAGTCATTCTCCATTAATCCAATTCATTGAGGTTTCCAGAAACAATA
GTCTTCCTGTGAGAAAATATGAATATTATATATGGTAAACATGATAATGG
AAAATTATTCAGAAAACTCTATCCCATGGAATCACATTCAAGGGTCAAGA
CCTCAAAGACAATCTAGGCTTCTTTTCTGAATTCTTGCTAAACAAGATAG
AATCATGCTAACAG
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no484_l20.b1 MSMg01no484_l20.b1 CHROMAT_FILE:
MSMg01no484_l20.b1 PHD_FILE: MSMg01no484_l20.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:06:25 2005
         (584 letters)



>mm_chr5_18840027_18841010
          Length = 984

 Score = 1091 bits (564), Expect = 0.0
 Identities = 579/584 (99%)
 Strand = Plus / Plus

                                                                       
Query: 1   tttacagatacatttgttcaatatgcctagtgtttattttctcctcaagggtagatataa 60
           |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 201 tttacagagacatttgttcaatatgcctagtgtttattttctcctcaagggtagatataa 260

                                                                       
Query: 61  cataaaaagctctgagagttgaggagttcgtagatcaatcctcctatttgaaaattaatc 120
           ||||||| |||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 261 cataaaaggctctgagagttgaggagttcggagatcaatcctcctatttgaaaattaatc 320

                                                                       
Query: 121 atcactctgaataggagacctgttggatattgtgtatacatttagtttcaaaatgattta 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 321 atcactctgaataggagacctgttggatattgtgtatacatttagtttcaaaatgattta 380

                                                                       
Query: 181 ctgtttctcttaatgttaccttgtactgattgaaactgcagaactaatggattactggag 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 ctgtttctcttaatgttaccttgtactgattgaaactgcagaactaatggattactggag 440

                                                                       
Query: 241 agtgaagcttcccctcttatctcaatcttggctgctgatgtagatttttgtgtagcctgt 300
           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 441 agtgaagcttctcctcttatctcaatcttggctgctgatgtagatttttgtgtagcctgt 500

                                                                       
Query: 301 gaagtttgcaaaacagcaggctagtcattagaaattgcagagtacagatcaaattcaaca 360
           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 501 gaagtttgcaaaacagcaggctattcattagaaattgcagagtacagatcaaattcaaca 560

                                                                       
Query: 361 gtcaatcactctactataacaattttattaagctctaactgaatctataataaatatact 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 561 gtcaatcactctactataacaattttattaagctctaactgaatctataataaatatact 620

                                                                       
Query: 421 attgtaagtctccagtatgttaccttgtgaattaaaaccatgctgaacagatccagtttt 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 621 attgtaagtctccagtatgttaccttgtgaattaaaaccatgctgaacagatccagtttt 680

                                                                       
Query: 481 catactctgattgtacaaatcctttgaacattaacatccaaataggggaatgccagggcc 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 681 catactctgattgtacaaatcctttgaacattaacatccaaataggggaatgccagggcc 740

                                                       
Query: 541 aggaagcaggagtgggtgggttggggaacaggggagggaggagg 584
           ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 741 aggaagcaggagtgggtgggttggggaacaggggagggaggagg 784


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 28
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 584
Length of database: 984
Length adjustment: 10
Effective length of query: 574
Effective length of database: 974
Effective search space:   559076
Effective search space used:   559076
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no484_l20.g1 MSMg01no484_l20.g1 CHROMAT_FILE:
MSMg01no484_l20.g1 PHD_FILE: MSMg01no484_l20.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:06:25 2005
         (691 letters)



>mm_chr5_19021375_19022465
          Length = 1091

 Score = 1266 bits (655), Expect = 0.0
 Identities = 682/691 (98%)
 Strand = Plus / Minus

                                                                       
Query: 1   aagtttgggcccagctttgctgatgagtaattagaaaaagtcttactgtctctctgcttt 60
           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 891 aagtttgggcccagccttgctgatgagtaattagaaaaagtcttactgtctctctgcttt 832

                                                                       
Query: 61  tctgagtagagaatcttagaaattaattctgtggaggaatgatgaaatcaaaagattcta 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 831 tctgagtagagaatcttagaaattaattctgtggaggaatgatgaaatcaaaagattcta 772

                                                                       
Query: 121 acatttccagaaggagacggctttttggattccaacaaagtccactggcttccattcaga 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 771 acatttccagaaggagacggctttttggattccaacaaagtccactggcttccattcaga 712

                                                                       
Query: 181 tgaatgcaatttcatattcccatttgagaataactatgagacaagattagttttaaaagt 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 711 tgaatgcaatttcatattcccatttgagaataactatgagacaagattagttttaaaagt 652

                                                                       
Query: 241 gtttattttaaaaaatttccaacatattgaagagagtgattgaaacatgtagctgtgctt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 651 gtttattttaaaaaatttccaacatattgaagagagtgattgaaacatgtagctgtgctt 592

                                                                       
Query: 301 tcaacaggagattatgatggtagcaagggatgcctggaccatggcaggccactgctgcca 360
           ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 591 tcaacaggagattatgatggtagcaagggatgcctggaccacggcaggccactgctgcca 532

                                                                       
Query: 361 gcaccaccttcctttacattagagttgggtaccagatcagagatatttttagctaacaac 420
           ||||||||||||||||||  |||||||||||||| ||| |||||||||||||||||||||
Sbjct: 531 gcaccaccttcctttacaacagagttgggtaccaaatctgagatatttttagctaacaac 472

                                                                       
Query: 421 agactattatatagctagtacccaatgatgttttctaaaggaatcaaataattgataaat 480
           |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 471 agactattatatagctagtacccaatgatgctttctaaaggaatcaaataattgataaat 412

                                                                       
Query: 481 gagtcattctccattaatccaattcattgaggtttccagaaacaatagtcttcctgtgag 540
           |||||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||
Sbjct: 411 gagtcattctccattaatccaattcattgaggcttccagaaacaataatcttcctgtgag 352

                                                                       
Query: 541 aaaatatgaatattatatatggtaaacatgataatggaaaattattcagaaaactctatc 600
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 351 aaaatatgaatattatatatggtaaacatgataatggaaaattattcagaaaactctatc 292

                                                                       
Query: 601 ccatggaatcacattcaagggtcaagacctcaaagacaatctaggcttcttttctgaatt 660
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 291 ccatggaatcacattcaagggtcaagacctcaaagacaatctaggcttcttttctgaatt 232

                                          
Query: 661 cttgctaaacaagatagaatcatgctaacag 691
           |||||||||||||||||||||||||||||||
Sbjct: 231 cttgctaaacaagatagaatcatgctaacag 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 31
Number of extensions: 2
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 691
Length of database: 1091
Length adjustment: 10
Effective length of query: 681
Effective length of database: 1081
Effective search space:   736161
Effective search space used:   736161
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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