BAC Information

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BAC

Clone NameMSMg01-517K24
Chromosome6 (Build37)
Map Location 32,176,503 - 32,238,373
singlet/doubletdoublet
Overlap genePlxna4
Upstream geneMkln1, Podxl, EG619959, LOC620104, 1700012A03Rik
Downstream geneChchd3, LOC100039118, Gm1401, Exoc4
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-517K24.T7 (MSMg01no517_k24.b1)MSMg01-517K24.TJ (MSMg01no517_k24.g1)
ACCAG589376AG589377
length793800
length of high quality
(start - end)		480 (32 - 511)508 (67 - 574)
definitionCHROMAT_FILE: MSMg01no517_k24.b1 PHD_FILE: MSMg01no517_k24.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:49:51 2005CHROMAT_FILE: MSMg01no517_k24.g1 PHD_FILE: MSMg01no517_k24.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 20:49:51 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(32,176,503 - 32,176,977)(32,237,866 - 32,238,373)
sequence
GCGGCGCTAATACATTACTATAGTCAGAGGATCCGCGGAATTCCATACTC
CTCTGAACAATCTGACAGACCCCTTCCTAAAGGAGAAACTGAGTCAGGGT
GAGGAAAAAAAGTACTCAATGTTGGAAGATGGGTATCTTTCCTCCCTTTT
GTGAAAGAACAGGTCCTGGTGAGGAAAACCCCCTCTGAATGGAATCTCTC
AGCCAGATGGAGCAGTGACATCCATCTGAGCAGAGCTGGAGCCCTGCTCT
GTCCAGAAGATGGCAAGAGCCAAAGCCTTCCTCACTTGTCCCTGTAAGAT
TCTCACTGAAGCTTGTTAGGAGTGCTCTAATGAGTCCTGATTAGCAGTGG
CCCCATAAAGGCCTAGTTTGCATATAAAAAGGTTGGCAGTTCTGAGAGGA
AGTTCATTAATAATAATTGGAGAGGCCCAGGGATGGGCTGGACACATCTT
CCTCTAAGTAGTGCTGATGTTTGACCCCTGTGCCCCTGCTTAATTGAAAT
GTAAAACGGCAATTTTCCCCTAGGCAACAAGGCATGAAGAGGGCATCTCC
ACCACCCTGCCCTTCCTCCCACCTATCATCCCAGACTGACCTGCTGTCTG
CTAAAATGCCAGGTGCAAAGCCACCTATAGACTCATGGGGAATGTCAGTT
TGCACAGTCCTCAAAAAAAAAAAAACTATTTGTCAGCCCAGGGGCTTANG
GACTCTGACATTTGGGNTCTGTGGCTANANAAGATGCTGGGAAGTATAAA
AATGAGTTGGTGAGNCCGCGTTTCCTCCTCTCACTCATTAAAA

TGGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXAACAGCTTGGGATGTCCCCTATCCCACCCAACCA
CTTTTATTACAAGAATCTTGCCTCCCAGTCCCTTGATTATCACTCTTTAA
AACTTGACATGAGAGAAGAAAGCCTTTCTCTTAACTGTGCCTGGGGGCTT
TGTGCCACTGGTGGGAGTGTGGGAGGGTAAGGCATGGAGGGTCTTCTGAA
TCTCTTGCATAGCAGAGGAGATTCCAGGATGTGGACTGCACATAAGAGAC
CTGTTGCTTATTTTGGGTTTACCCAACAGCTTGTATGGCACCTGTACCAT
GGCAATCTTTCTGCCTAGAAAATAACTCTTATTAGCAGATTCTGCATGTG
TTTATTTTGTTGCATCAAGTCTGATCTTTCTTTTAAGTGAGATTCACTGT
CATTTGGTGGTATCCTTTCAACTCAATTCAGTATGTGCTGCACAGTTCAC
CTATTAGAGGCACTGCCATGGGACAGCACAAATTTGATCAAACAAAGGCT
TTTGTGAGTAAAATTTCATTGTAGCTTGGTCATCACCATATATTTGCATA
TACTGTGTGGCTCTGTTTACCACAGAAACAGGGTGGCATAATTTATACTA
AGTCTAAAATATAAACTATCTTTACTATAACTCGATATGGCTTTTCCGGG
CTTAGGGGTATTATTAAATAGTAGAATGAAGTCGGAACAGGGTCCTCATC
CTCTGGGGAGGTATGTGGTTATGTTGCAAAAGACCCTAAACCGTTAAGAA
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no517_k24.b1 MSMg01no517_k24.b1 CHROMAT_FILE:
MSMg01no517_k24.b1 PHD_FILE: MSMg01no517_k24.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:49:51 2005
         (480 letters)



>mm_chr6_32176298_32177177
          Length = 880

 Score =  919 bits (475), Expect = 0.0
 Identities = 475/475 (100%)
 Strand = Plus / Plus

                                                                       
Query: 6   ggaattccatactcctctgaacaatctgacagaccccttcctaaaggagaaactgagtca 65
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 206 ggaattccatactcctctgaacaatctgacagaccccttcctaaaggagaaactgagtca 265

                                                                       
Query: 66  gggtgaggaaaaaaagtactcaatgttggaagatgggtatctttcctcccttttgtgaaa 125
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 266 gggtgaggaaaaaaagtactcaatgttggaagatgggtatctttcctcccttttgtgaaa 325

                                                                       
Query: 126 gaacaggtcctggtgaggaaaaccccctctgaatggaatctctcagccagatggagcagt 185
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 326 gaacaggtcctggtgaggaaaaccccctctgaatggaatctctcagccagatggagcagt 385

                                                                       
Query: 186 gacatccatctgagcagagctggagccctgctctgtccagaagatggcaagagccaaagc 245
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 386 gacatccatctgagcagagctggagccctgctctgtccagaagatggcaagagccaaagc 445

                                                                       
Query: 246 cttcctcacttgtccctgtaagattctcactgaagcttgttaggagtgctctaatgagtc 305
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 446 cttcctcacttgtccctgtaagattctcactgaagcttgttaggagtgctctaatgagtc 505

                                                                       
Query: 306 ctgattagcagtggccccataaaggcctagtttgcatataaaaaggttggcagttctgag 365
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 506 ctgattagcagtggccccataaaggcctagtttgcatataaaaaggttggcagttctgag 565

                                                                       
Query: 366 aggaagttcattaataataattggagaggcccagggatgggctggacacatcttcctcta 425
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 566 aggaagttcattaataataattggagaggcccagggatgggctggacacatcttcctcta 625

                                                                  
Query: 426 agtagtgctgatgtttgacccctgtgcccctgcttaattgaaatgtaaaacggca 480
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 626 agtagtgctgatgtttgacccctgtgcccctgcttaattgaaatgtaaaacggca 680


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 24
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 480
Length of database: 880
Length adjustment: 10
Effective length of query: 470
Effective length of database: 870
Effective search space:   408900
Effective search space used:   408900
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no517_k24.g1 MSMg01no517_k24.g1 CHROMAT_FILE:
MSMg01no517_k24.g1 PHD_FILE: MSMg01no517_k24.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 20:49:51 2005
         (508 letters)



>mm_chr6_32237666_32238573
          Length = 908

 Score =  967 bits (500), Expect = 0.0
 Identities = 506/508 (99%)
 Strand = Plus / Minus

                                                                       
Query: 1   aacagcttgggatgtcccctatcccacccaaccacttttattacaagaatcttgcctccc 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 708 aacagcttgggatgtcccctatcccacccaaccacttttattacaagaatcttgcctccc 649

                                                                       
Query: 61  agtcccttgattatcactctttaaaacttgacatgagagaagaaagcctttctcttaact 120
           ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 648 agtccctcgattatcactctttaaaacttgacatgagagaagaaagcctttctcttaact 589

                                                                       
Query: 121 gtgcctgggggctttgtgccactggtgggagtgtgggagggtaaggcatggagggtcttc 180
           |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct: 588 gtgcctgggggctttgtgccactggtgggagtatgggagggtaaggcatggagggtcttc 529

                                                                       
Query: 181 tgaatctcttgcatagcagaggagattccaggatgtggactgcacataagagacctgttg 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 528 tgaatctcttgcatagcagaggagattccaggatgtggactgcacataagagacctgttg 469

                                                                       
Query: 241 cttattttgggtttacccaacagcttgtatggcacctgtaccatggcaatctttctgcct 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 468 cttattttgggtttacccaacagcttgtatggcacctgtaccatggcaatctttctgcct 409

                                                                       
Query: 301 agaaaataactcttattagcagattctgcatgtgtttattttgttgcatcaagtctgatc 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 408 agaaaataactcttattagcagattctgcatgtgtttattttgttgcatcaagtctgatc 349

                                                                       
Query: 361 tttcttttaagtgagattcactgtcatttggtggtatcctttcaactcaattcagtatgt 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 348 tttcttttaagtgagattcactgtcatttggtggtatcctttcaactcaattcagtatgt 289

                                                                       
Query: 421 gctgcacagttcacctattagaggcactgccatgggacagcacaaatttgatcaaacaaa 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 288 gctgcacagttcacctattagaggcactgccatgggacagcacaaatttgatcaaacaaa 229

                                       
Query: 481 ggcttttgtgagtaaaatttcattgtag 508
           ||||||||||||||||||||||||||||
Sbjct: 228 ggcttttgtgagtaaaatttcattgtag 201


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 26
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 508
Length of database: 908
Length adjustment: 10
Effective length of query: 498
Effective length of database: 898
Effective search space:   447204
Effective search space used:   447204
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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