BAC Information

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BAC

Clone NameMSMg01-543D02
Chromosome8 (Build37)
Map Location 65,525,224 - 65,626,292
singlet/doubletdoublet
Overlap geneSpock3
Upstream geneAnxa10, LOC100040775, LOC384830, LOC100040804, LOC665267
Downstream geneLOC384831, EG244495, Tll1
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-543D02.T7 (MSMg01no543_d02.b1)MSMg01-543D02.TJ (MSMg01no543_d02.g1)
ACCAG608788AG608789
length747748
length of high quality
(start - end)		661 (51 - 711)665 (81 - 745)
definitionCHROMAT_FILE: MSMg01no543_d02.b1 PHD_FILE: MSMg01no543_d02.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 21:23:28 2005CHROMAT_FILE: MSMg01no543_d02.g1 PHD_FILE: MSMg01no543_d02.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 21:23:29 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(65,525,224 - 65,525,880)(65,625,628 - 65,626,292)
sequence
CCGATTTATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
CGCGGAATTCAAGGTCTTAGCTTTCTAGGTAAGATCTCTACAACTGATCA
GAACCCCAAACTCATATCAGTATATACTTTCATAGATAATCATCTTTTAA
ATAAAAATAGAACTTATTAGATTTTATAATTTTAATATTTCTAATGACAT
ATTTATATCATTTTATTATTCAATCATTTACTTGTAATACTTATATTTTG
TTGACTATAAGACAAAGCTAATTTTAATATGGCTTTCTGAGAGAAAAATT
ATCAGTTATACCTTATAAATATTGAGATGTCCCATTTGAGTTAACTGCTT
TTAGCTGTTAAGATTTTACTTTTAATATAATATCGGTTTCGATAGAAATG
AGTATCATATTTATTTACGAATTGCTGGTAGAAAGGTGTTTATAGTAATT
ATAAAAGCTTTTAAGCTGAAATTTTCCTTTACTTAGATTTTTCTAAGGTT
TCATTAGATTTAAATATTGGCAATGACTGAACTATCAGGAAATTGTAGGC
ATTATATTTAATCATTTGACAAATCTATTACCTCTGTAGGGTTGAGTAAC
TGATATTTTTTGTGTTAATGGTTCATGGTTAGATAAGATATGATTCTTAG
CTTGGAAAATAGAAACCACAAATATTAATATAATTTAAATATATTCATGC
ATATTAGAAAATTGTATATTAAATTCTCCAATATCAGTAATTAAACA

NCCACGTATGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGTGAACATTATGAAAGAAG
AAGTTTGAGACTAAAGCAAATGAGAAGATGTTGTAATGTAACCTTTGGAA
AGGGAATTAGGAAAAAGATCTTTGAATAACAAGACTTTCCCAACACTTTA
GGCTACAGACAGGGGATCCATTTCTTTTTAGTGTTAGGATAAGATAATAT
CAGTATAAAAATCTGATATTATAGTTTCTATCTCTTTTCATTAACTATTA
TACCTCAGCATGTTCTCATACCTCCAAATGTAATCTGTTATCTCCCATAT
TAGCTTTGTCATTGATCAACCTGCATGCCAAATTTCTAGTTCCTAAAGGG
CTGTATACACATGGAGTTTGTGCTAACTTCTCAAGTCATATAAAATATTA
ATCTGCTGTCACCTGAACACTAAATACAGTTTACATAAAACACTGAAACT
TGCCAGTTTAAATTCTAGAGATCTTTCTCATTCTACTGGTCACTAAAGGA
AATTCAGCTGCTACTTCTCAAGGTGGTTTAATGGCTTTCACTAAGCAATA
TTTGTGCTTTTTGTTTCTGACCATCCCTAACATGGAAAATGGAAACCAAG
TTTCCTTTTGTATGTAGTTTATTAAAATTATCTTTAAATTTATTTACATA
TTTATGTATTTATTTATTTATCAATTGATTAATTGATTTTGTGAGGGG
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no543_d02.b1 MSMg01no543_d02.b1 CHROMAT_FILE:
MSMg01no543_d02.b1 PHD_FILE: MSMg01no543_d02.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 21:23:28 2005
         (661 letters)



>mm_chr8_65525020_65526080
          Length = 1061

 Score = 1239 bits (641), Expect = 0.0
 Identities = 653/657 (99%)
 Strand = Plus / Plus

                                                                       
Query: 5   gaattcaaggtcttagctttctaggtaagatctctacaactgatcagaaccccaaactca 64
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 205 gaattcaaggtcttagctttctaggtaagatctctacaactgatcagaaccccaaactca 264

                                                                       
Query: 65  tatcagtatatactttcatagataatcatcttttaaataaaaatagaacttattagattt 124
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 265 tatcagtatatactttcatagataatcatcttttaaataaaaatagaacttattagattt 324

                                                                       
Query: 125 tataattttaatatttctaatgacatatttatatcattttattattcaatcatttacttg 184
           ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct: 325 tataattttaatatttctaatgacataattatatcattttattattcaatcatttacttg 384

                                                                       
Query: 185 taatacttatattttgttgactataagacaaagctaattttaatatggctttctgagaga 244
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 385 taatacttatattttgttgactataagacaaagctaattttaatatggctttctgagaga 444

                                                                       
Query: 245 aaaattatcagttataccttataaatattgagatgtcccatttgagttaactgcttttag 304
           |||||||||||||||||||||||||| |||| ||||||||||||||||||||||||||||
Sbjct: 445 aaaattatcagttataccttataaatgttgacatgtcccatttgagttaactgcttttag 504

                                                                       
Query: 305 ctgttaagattttacttttaatataatatcggtttcgatagaaatgagtatcatatttat 364
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 505 ctgttaagattttacttttaatataatatcggtttcgatagaaatgagtatcatatttat 564

                                                                       
Query: 365 ttacgaattgctggtagaaaggtgtttatagtaattataaaagcttttaagctgaaattt 424
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 565 ttacgaattgctggtagaaaggtgtttatagtaattataaaagcttttaagctgaaattt 624

                                                                       
Query: 425 tcctttacttagatttttctaaggtttcattagatttaaatattggcaatgactgaacta 484
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 625 tcctttacttagatttttctaaggtttcattagatttaaatattggcaatgactgaacta 684

                                                                       
Query: 485 tcaggaaattgtaggcattatatttaatcatttgacaaatctattacctctgtagggttg 544
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 685 tcaggaaattgtaggcattatatttaatcatttgacaaatctattacctctgtagggttg 744

                                                                       
Query: 545 agtaactgatattttttgtgttaatggttcatggttagataagatatgattcttagcttg 604
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 745 agtaactgatattttttgtgttaatggttcatggttagataagatatgattcttagcttg 804

                                                                    
Query: 605 gaaaatagaaaccacaaatattaatataatttaaatatattcatgcatattagaaaa 661
           |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct: 805 gaaaatagaaaccacaaatattaatataatttaaatatattcattcatattagaaaa 861


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 35
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 661
Length of database: 1061
Length adjustment: 10
Effective length of query: 651
Effective length of database: 1051
Effective search space:   684201
Effective search space used:   684201
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no543_d02.g1 MSMg01no543_d02.g1 CHROMAT_FILE:
MSMg01no543_d02.g1 PHD_FILE: MSMg01no543_d02.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 21:23:29 2005
         (665 letters)



>mm_chr8_65625486_65626550
          Length = 1065

 Score = 1224 bits (633), Expect = 0.0
 Identities = 657/665 (98%)
 Strand = Plus / Minus

                                                                       
Query: 1   tgtgaacattatgaaagaagaagtttgagactaaagcaaatgagaagatgttgtaatgta 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 807 tgtgaacattatgaaagaagaagtttgagactaaagcaaatgagaagatgttgtaatgta 748

                                                                       
Query: 61  acctttggaaagggaattaggaaaaagatctttgaataacaagactttcccaacacttta 120
           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct: 747 acctttggaaagggaattagggaaaagatctttgaataacaagactttcccaacacttta 688

                                                                       
Query: 121 ggctacagacaggggatccatttctttttagtgttaggataagataatatcagtataaaa 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 687 ggctacagacaggggatccatttctttttagtgttaggataagataatatcagtataaaa 628

                                                                       
Query: 181 atctgatattatagtttctatctcttttcattaactattatacctcagcatgttctcata 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 627 atctgatattatagtttctatctcttttcattaactattatacctcagcatgttctcata 568

                                                                       
Query: 241 cctccaaatgtaatctgttatctcccatattagctttgtcattgatcaacctgcatgcca 300
           |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 567 cctccaaatgcaatctgttatctcccatattagctttgtcattgatcaacctgcatgcca 508

                                                                       
Query: 301 aatttctagttcctaaagggctgtatacacatggagtttgtgctaacttctcaagtcata 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 507 aatttctagttcctaaagggctgtatacacatggagtttgtgctaacttctcaagtcata 448

                                                                       
Query: 361 taaaatattaatctgctgtcacctgaacactaaatacagtttacataaaacactgaaact 420
           |||||||||||| | |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 447 taaaatattaatatcctgtcacctgaacactaaatacagtttacataaaacactgaaact 388

                                                                       
Query: 421 tgccagtttaaattctagagatctttctcattctactggtcactaaaggaaattcagctg 480
           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct: 387 tgccagtttaaattctagagatctttctcattctaatggtcactaaaggaaattcagctg 328

                                                                       
Query: 481 ctacttctcaaggtggtttaatggctttcactaagcaatatttgtgctttttgtttctga 540
           ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct: 327 ctacttctcaaggtggtttaatggctttcactaagcaatatttgtactttttgtttctga 268

                                                                       
Query: 541 ccatccctaacatggaaaatggaaaccaagtttccttttgtatgtagtttattaaaatta 600
           |||||| || ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 267 ccatccttaccatggaaaatggaaaccaagtttccttttgtatgtagtttattaaaatta 208

                                                                       
Query: 601 tctttaaatttatttacatatttatgtatttatttatttatcaattgattaattgatttt 660
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 207 tctttaaatttatttacatatttatgtatttatttatttatcaattgattaattgatttt 148

                
Query: 661 gtgag 665
           |||||
Sbjct: 147 gtgag 143


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 37
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 665
Length of database: 1065
Length adjustment: 10
Effective length of query: 655
Effective length of database: 1055
Effective search space:   691025
Effective search space used:   691025
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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