BAC Information

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BAC

Clone NameMSMg01-409L13
Chromosome5 (Build37)
Map Location 131,252,736 - 131,400,139
singlet/doubletdoublet
Overlap geneCaln1, Wbscr17
Upstream geneGbas, Psph, Cct6a, LOC100038887, Sumf2, Phkg1, Chchd2, 2410018M08Rik, 4930579G22Rik, Vkorc1l1, Gusb, Asl, Crcp, Tpst1, Kctd7, Rabgef1, 0610007L01Rik, EG625540, Sbds, Tyw1, A330070K13Rik
Downstream geneAuts2, 4930563F08Rik
LinkOpen Mouse BAC browser



BAC End Information

BAC end BAC-end 1BAC-end 2
end nameMSMg01-409L13.T7 (MSMg01no409_l13.b1)MSMg01-409L13.TJ (MSMg01no409_l13.g1)
ACCAG507139AG507140
length779739
length of high quality
(start - end)		422 (72 - 493)337 (74 - 410)
definitionCHROMAT_FILE: MSMg01no409_l13.b1 PHD_FILE: MSMg01no409_l13.b1.phd.1 CHEM: term DYE: big TIME: Wed May 18 18:26:11 2005CHROMAT_FILE: MSMg01no409_l13.g1 PHD_FILE: MSMg01no409_l13.g1.phd.1 CHEM: term DYE: big TIME: Wed May 18 18:26:11 2005
singlet/doubletdoubletdoublet
BLAST hituniqueunique
Map location.(131,252,736 - 131,253,157)(131,399,803 - 131,400,139)
sequence
NAATCATXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTCGAGAGAATCCGC
GGAATTCCTTCTCTGTCACGCTCAGTTGTGTGGATCAAGTTGATAGATAA
ATCCAACTAGAGCATGCTTCATTTAGTACAACCTCCAGATGTTACTGGAA
ATAAACACAGCAAGATGAAACTGAACCTGCCTAGTTTCTTATTCAATTTG
AAAAAGCGTCAACATCTATGATAATGTTTACTGATATTTAAAGCTAAGAG
GTGTTTTCAATCGACATACACTTGATCAAGTATCTCTGTTGTAGGATATG
AAACCCATATGTGGTATAGTGTTTATTTGTCCAGGACTGGCAGAGCTGGG
TCATAGGATAGTTCAATTGTTGTTTTATTTTACAATCCTAATATGCCTAA
TATAAAAGACACACAATCAAGATTCTTTCATTATAAGCCATATGCCTAGA
TTGGGCCCATCTAGGACTATACTAAGTTATTCCCCAGCTTTAAAAAACTT
TGTTGCTTGCTGTTTCTCATGGACATATGCTTCTGGTCCATGGTGGGTTT
CTCCTTCATCCTCTTTCTCTCTATAGCCATGCGCCCTTCAAATCCAGTCC
CCCCTTGGTTGTTTGGTTGGTAACACCCTAATTGATTTCTAAATACTTGG
ACCATTTTTTTTTCTTTTTATTACTCCTATAGTTCCCCTTGCCCCATACT
AAACCTTCCATATTTTGCGGGTTATCATCTTCTTCCCTACCGCTTTACCA
CGTCTAACCCCCCTCCCATTTCTCATCCA

ATGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXATTCCCAATACTATAGTGGCCTGAGCA
TTACAAACAGGAAGACAAAAGGATAGGAAGGTGGGCTCCTAAGTGCTTTG
TCACTCTTGCAAAATACATGAAGTTTATTTTCACTTCGTGTTTTCAGATT
TCACTTCATAGTCCATTGATCTGTGGGCGAGCCTCAAGAACATTTTCTTG
ACTAATGATTAATATGGGATGGCCCTGCCTCCTGGAGGCAATGCCACCCC
AGGGTCCATTGTCCTGGGGGTGTTTAAGAAAATGGGGTGAACAAGCCTGT
GTTCTTCCATGTCCTTTGCATCAGTTCCAGCCTTGGGTTCCTGCCTTGAC
TTTCCTGGATTGAACGACCACAGGTATAACATGAATACTCCTCCTCCCTG
ATCTTTTTTTTGTCTCCCAAGATATTTTTGGTCCTTATGCCATTCCCTGG
GCATATAATTAGTGTCCTTTCAATACTGGAACCTATTGACCCCTCTGGTC
TGCATTTCTTCTCGTCTCTTTTACCTATTAACTTAGTTACGTTGCTTTCC
TCACTCTTTATGTTCTCTCGCTCTTCAATCGCATTGTAATTGTTTCCCCC
TTTTTATTACATTTAGCTTTACGCTATAAACTTTCATTTTTTATATTCTT
TCTTCTTTTTACAGTGTCCCTCCGTTTACTTAAGGGCCT
quality valuesOpen.Open.




Alignment Information

BAC End 1BAC End 2
Query= MSMg01no409_l13.b1 MSMg01no409_l13.b1 CHROMAT_FILE:
MSMg01no409_l13.b1 PHD_FILE: MSMg01no409_l13.b1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 18:26:11 2005
         (422 letters)



>mm_chr5_131252536_131253357
          Length = 822

 Score =  746 bits (386), Expect = 0.0
 Identities = 413/422 (97%)
 Strand = Plus / Plus

                                                                       
Query: 1   tcagttgtgtggatcaagttgatagataaatccaactagagcatgcttcatttagtacaa 60
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct: 201 tcagttgtgtggatcaagttgatagataaatccaactagagcatgcttcatttagtgcaa 260

                                                                       
Query: 61  cctccagatgttactggaaataaacacagcaagatgaaactgaacctgcctagtttctta 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 261 cctccagatgttactggaaataaacacagcaagatgaaactgaacctgcctagtttctta 320

                                                                       
Query: 121 ttcaatttgaaaaagcgtcaacatctatgataatgtttactgatatttaaagctaagagg 180
           | |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 321 tccaatttgaaaaagcgtcaacatctatgacaatgtttactgatatttaaagctaagagg 380

                                                                       
Query: 181 tgttttcaatcgacatacacttgatcaagtatctctgttgtaggatatgaaacccatatg 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 381 tgttttcaatcgacatacacttgatcaagtatctctgttgtaggatatgaaacccatatg 440

                                                                       
Query: 241 tggtatagtgtttatttgtccaggactggcagagctgggtcataggatagttcaattgtt 300
           ||||||| ||  ||| ||||||||||||| ||||||||||||||||||||||||||||||
Sbjct: 441 tggtatattgggtatatgtccaggactggtagagctgggtcataggatagttcaattgtt 500

                                                                       
Query: 301 gttttattttacaatcctaatatgcctaatataaaagacacacaatcaagattctttcat 360
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 501 cttttattttacaatcctaatatgcctaatataaaagacacacaatcaagattctttcat 560

                                                                       
Query: 361 tataagccatatgcctagattgggcccatctaggactatactaagttattccccagcttt 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 561 tataagccatatgcctagattgggcccatctaggactatactaagttattccccagcttt 620

             
Query: 421 aa 422
           ||
Sbjct: 621 aa 622


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 20
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 422
Length of database: 822
Length adjustment: 9
Effective length of query: 413
Effective length of database: 813
Effective search space:   335769
Effective search space used:   335769
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)

Query= MSMg01no409_l13.g1 MSMg01no409_l13.g1 CHROMAT_FILE:
MSMg01no409_l13.g1 PHD_FILE: MSMg01no409_l13.g1.phd.1 CHEM: term DYE:
big TIME: Wed May 18 18:26:11 2005
         (337 letters)



>mm_chr5_131399799_131400535
          Length = 737

 Score =  621 bits (321), Expect = 0.0
 Identities = 333/337 (98%)
 Strand = Plus / Minus

                                                                       
Query: 1   attcccaatactatagtggcctgagcattacaaacaggaagacaaaaggataggaaggtg 60
           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct: 341 attcccaatactatagtggcctgagcattacaaacgggaagacaaaaggataggaaggtg 282

                                                                       
Query: 61  ggctcctaagtgctttgtcactcttgcaaaatacatgaagtttattttcacttcgtgttt 120
           |||||||||||||||||||| |||||||||||||||||||||||||||| ||||||||||
Sbjct: 281 ggctcctaagtgctttgtcattcttgcaaaatacatgaagtttattttcgcttcgtgttt 222

                                                                       
Query: 121 tcagatttcacttcatagtccattgatctgtgggcgagcctcaagaacattttcttgact 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 221 tcagatttcacttcatagtccattgatctgtgggcgagcctcaagaacattttcttgact 162

                                                                       
Query: 181 aatgattaatatgggatggccctgcctcctggaggcaatgccaccccagggtccattgtc 240
           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 161 aatgattaatatggaatggccctgcctcctggaggcaatgccaccccagggtccattgtc 102

                                                                       
Query: 241 ctgggggtgtttaagaaaatggggtgaacaagcctgtgttcttccatgtcctttgcatca 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 101 ctgggggtgtttaagaaaatggggtgaacaagcctgtgttcttccatgtcctttgcatca 42

                                                
Query: 301 gttccagccttgggttcctgccttgactttcctggat 337
           |||||||||||||||||||||||||||||||||||||
Sbjct: 41  gttccagccttgggttcctgccttgactttcctggat 5


Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.34    0.600     1.10 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 2, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 14
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 337
Length of database: 737
Length adjustment: 9
Effective length of query: 328
Effective length of database: 728
Effective search space:   238784
Effective search space used:   238784
X1: 8 (15.9 bits)
X2: 517 (999.5 bits)
X3: 517 (999.5 bits)
S1: 8 (16.4 bits)
S2: 8 (16.2 bits)


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